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Literature summary extracted from

  • Ivanov, I.; Romanov, S.; Ozdoba, C.; Holzhutter, H.G.; Myagkova, G.; Kuhn, H.
    Enantioselective substrate specificity of 15-lipoxygenase 1 (2004), Biochemistry, 43, 15720-15728.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.33 the recombinant wild-type enzyme and various mutants are expressed in Escherichia coli as His-tagged fusion proteins Oryctolagus cuniculus

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.33 F353L the ratio of turnover-number to KM-value is 4fold lower than the value for the wild-type enzyme. The mutation strongly alters the positional specificity of the oxygenation of most substrates in favor of 12-lipoxygenation except from (16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid Oryctolagus cuniculus
1.13.11.33 I593A mutation induces alterations in the stereochemical characteristics of hydroxy fatty acid oxygenation Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.33 0.00301
-
arachidonic acid pH 7.4, wild-type enzyme Oryctolagus cuniculus
1.13.11.33 0.0087
-
arachidonic acid pH 7.4, mutant enzyme F353L Oryctolagus cuniculus
1.13.11.33 0.0227
-
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, mutant enzyme F353L Oryctolagus cuniculus
1.13.11.33 0.0236
-
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, wild-type enzyme Oryctolagus cuniculus
1.13.11.33 0.0289
-
(16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, wild-type enzyme Oryctolagus cuniculus
1.13.11.33 0.0313
-
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, mutant enzyme L353L Oryctolagus cuniculus
1.13.11.33 0.0368
-
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, mutant enzyme L353L Oryctolagus cuniculus
1.13.11.33 0.136
-
(16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, mutant enzyme F353L Oryctolagus cuniculus
1.13.11.33 0.236
-
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, wild-type enzyme Oryctolagus cuniculus
1.13.11.33 0.293
-
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, wild-type enzyme Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.33 Oryctolagus cuniculus P12530
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.33
-
Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.33 additional information stroma-free hemolysis supernatant of a reticulocyte-rich blood cell suspension Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.33 (16(R),5Z,8Z,11Z,14Z)-16-fluoroeicosa-5,8,11,14-tetraenoic acid + O2
-
Oryctolagus cuniculus ? wild-type enzyme: 78% of the 15,16(R) product and 22% of the 12,16(R) product ?
1.13.11.33 (16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2
-
Oryctolagus cuniculus ? wild-type enzyme and mutant enzyme I593A prouce small amounts of unspecific products. Mutant enzyme F353L produces 6% of 15,16(R) product and 94% of the 12,16(R) product ?
1.13.11.33 (16(S),5Z,8Z,11Z,14Z)-16-fluoroeicosa-5,8,11,14-tetraenoic acid + O2
-
Oryctolagus cuniculus ? wild-type enzyme: 69% of the 15,16(S) product and 31% of the 12,16(S) product ?
1.13.11.33 (16(S)5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2
-
Oryctolagus cuniculus ? wild-type enzyme: 93% of the 15,16(S) product and 7% of the 12,16(S) product ?
1.13.11.33 (17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2
-
Oryctolagus cuniculus ? wild-type enzyme: 1% of the 15,17(R) product and 99% of the 12,17(R) product ?
1.13.11.33 (17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2
-
Oryctolagus cuniculus ? wild-type enzyme: 3% of the 15,17(S) product and 97% of the 12,17(S) product ?
1.13.11.33 (18(R),5Z,8Z,11Z,14Z)-18-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 oxygenation proceeds with little if any enantioselectivity Oryctolagus cuniculus ?
-
?
1.13.11.33 (18(S),5Z,8Z,11Z,14Z)-18-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 oxygenation proceeds with little if any enantioselectivity Oryctolagus cuniculus ?
-
?
1.13.11.33 arachidonic acid + O2
-
Oryctolagus cuniculus (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid + (12S,5Z,8Z,10E,14Z)-12-hydroperoxyeicosa-5,8,10,14-tetraenoic acid 93% (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid and 7% (12S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.33 15-lipoxygenase 1
-
Oryctolagus cuniculus
1.13.11.33 15-LOX
-
Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.33 0.08
-
(16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, mutant enzyme F353L Oryctolagus cuniculus
1.13.11.33 0.18
-
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, wild-type enzyme Oryctolagus cuniculus
1.13.11.33 0.2
-
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, mutant enzyme F353L Oryctolagus cuniculus
1.13.11.33 0.2
-
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, mutant enzyme L353L Oryctolagus cuniculus
1.13.11.33 0.24
-
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, mutant enzyme L353L Oryctolagus cuniculus
1.13.11.33 0.51
-
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, wild-type enzyme Oryctolagus cuniculus
1.13.11.33 1.24
-
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid pH 7.4, wild-type enzyme Oryctolagus cuniculus
1.13.11.33 6.02
-
arachidonic acid pH 7.4, mutant enzyme F353L Oryctolagus cuniculus
1.13.11.33 8.16
-
arachidonic acid pH 7.4, wild-type enzyme Oryctolagus cuniculus