EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.33 | the recombinant wild-type enzyme and various mutants are expressed in Escherichia coli as His-tagged fusion proteins | Oryctolagus cuniculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.11.33 | F353L | the ratio of turnover-number to KM-value is 4fold lower than the value for the wild-type enzyme. The mutation strongly alters the positional specificity of the oxygenation of most substrates in favor of 12-lipoxygenation except from (16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | Oryctolagus cuniculus |
1.13.11.33 | I593A | mutation induces alterations in the stereochemical characteristics of hydroxy fatty acid oxygenation | Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.33 | 0.00301 | - |
arachidonic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
1.13.11.33 | 0.0087 | - |
arachidonic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
1.13.11.33 | 0.0227 | - |
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
1.13.11.33 | 0.0236 | - |
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
1.13.11.33 | 0.0289 | - |
(16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
1.13.11.33 | 0.0313 | - |
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme L353L | Oryctolagus cuniculus | |
1.13.11.33 | 0.0368 | - |
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme L353L | Oryctolagus cuniculus | |
1.13.11.33 | 0.136 | - |
(16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
1.13.11.33 | 0.236 | - |
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
1.13.11.33 | 0.293 | - |
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.33 | Oryctolagus cuniculus | P12530 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.33 | - |
Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.33 | additional information | stroma-free hemolysis supernatant of a reticulocyte-rich blood cell suspension | Oryctolagus cuniculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.33 | (16(R),5Z,8Z,11Z,14Z)-16-fluoroeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme: 78% of the 15,16(R) product and 22% of the 12,16(R) product | ? | |
1.13.11.33 | (16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme and mutant enzyme I593A prouce small amounts of unspecific products. Mutant enzyme F353L produces 6% of 15,16(R) product and 94% of the 12,16(R) product | ? | |
1.13.11.33 | (16(S),5Z,8Z,11Z,14Z)-16-fluoroeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme: 69% of the 15,16(S) product and 31% of the 12,16(S) product | ? | |
1.13.11.33 | (16(S)5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme: 93% of the 15,16(S) product and 7% of the 12,16(S) product | ? | |
1.13.11.33 | (17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme: 1% of the 15,17(R) product and 99% of the 12,17(R) product | ? | |
1.13.11.33 | (17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme: 3% of the 15,17(S) product and 97% of the 12,17(S) product | ? | |
1.13.11.33 | (18(R),5Z,8Z,11Z,14Z)-18-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | oxygenation proceeds with little if any enantioselectivity | Oryctolagus cuniculus | ? | - |
? | |
1.13.11.33 | (18(S),5Z,8Z,11Z,14Z)-18-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | oxygenation proceeds with little if any enantioselectivity | Oryctolagus cuniculus | ? | - |
? | |
1.13.11.33 | arachidonic acid + O2 | - |
Oryctolagus cuniculus | (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid + (12S,5Z,8Z,10E,14Z)-12-hydroperoxyeicosa-5,8,10,14-tetraenoic acid | 93% (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid and 7% (12S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.33 | 15-lipoxygenase 1 | - |
Oryctolagus cuniculus |
1.13.11.33 | 15-LOX | - |
Oryctolagus cuniculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.33 | 0.08 | - |
(16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
1.13.11.33 | 0.18 | - |
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
1.13.11.33 | 0.2 | - |
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
1.13.11.33 | 0.2 | - |
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme L353L | Oryctolagus cuniculus | |
1.13.11.33 | 0.24 | - |
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme L353L | Oryctolagus cuniculus | |
1.13.11.33 | 0.51 | - |
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
1.13.11.33 | 1.24 | - |
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
1.13.11.33 | 6.02 | - |
arachidonic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
1.13.11.33 | 8.16 | - |
arachidonic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus |