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Literature summary extracted from

  • Rogge, C.E.; Liu, W.; Wu, G.; Wang, L.H.; Kulmacz, R.J.; Tsai, A.L.
    Identification of Tyr504 as an alternative tyrosyl radical site in human prostaglandin H synthase-2 (2004), Biochemistry, 43, 1560-1568.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.1 all mutants are overexpressed in the baculovirus system as detergent-soluble full-length proteins Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.99.1 Y148F mutant enzyme has cyclooxygenase activity comparable to that of the native enzyme Homo sapiens
1.14.99.1 Y148F/Y348F/Y385F/Y404F/Y504F no cyclooxygenase activity detected Homo sapiens
1.14.99.1 Y148F/Y348F/Y404F/Y504F mutant enzyme has specific cyclooxygenase activity approximately half that of native enzyme. Modest increase in cyclooxygenase self-inactivation rate, 2.3fold Homo sapiens
1.14.99.1 Y348F mutant enzyme has specific cyclooxygenase activity approximately half that of native enzyme Homo sapiens
1.14.99.1 Y385F no cyclooxygenase activity detected Homo sapiens
1.14.99.1 Y404F mutant enzyme has cyclooxygenase activity comparable to that of the native enzyme. Modest increase in cyclooxygenase self-inactivation rate, 2.3fold Homo sapiens
1.14.99.1 Y504F mutant enzyme has cyclooxygenase activity comparable to that of the native enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.1 0.0021
-
 arachidonate native enzyme Homo sapiens
1.14.99.1 0.0032
-
 arachidonate mutant enzyme Y148F Homo sapiens
1.14.99.1 0.0036
-
 arachidonate mutant enzyme Y404F Homo sapiens
1.14.99.1 0.0038
-
 arachidonate mutant enzyme Y348F Homo sapiens
1.14.99.1 0.0042
-
 arachidonate mutant enzyme Y504F Homo sapiens
1.14.99.1 0.0043
-
 arachidonate mutant enzyme Y148F/Y348F/Y404F/Y504F Homo sapiens
1.14.99.1 0.02
-
 trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y148F Homo sapiens
1.14.99.1 0.061
-
 trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y404F Homo sapiens
1.14.99.1 0.072
-
 trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y348F Homo sapiens
1.14.99.1 0.103
-
 trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y148F/Y348F/Y385F/Y404F/Y504F Homo sapiens
1.14.99.1 0.138
-
 trans-5-phenyl-4-pentenyl-1-hydroperoxide native enzyme Homo sapiens
1.14.99.1 0.339
-
 trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y385F Homo sapiens
1.14.99.1 0.37
-
 trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y148F/Y348F/Y404F/Y504F Homo sapiens
1.14.99.1 0.437
-
 trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y504F Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.1 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.1 arachidonate + electron donor + O2 cyclooxygenase activity Homo sapiens prostaglandin H2 + oxidized electron donor + H2O
-
 ?
1.14.99.1 guaiacol + trans-5-phenyl-4-pentenyl-1-hydroperoxide
-
 Homo sapiens ?
-
 ?
1.14.99.1 H2O2 + N,N,N',N'-tetramethyl-p-phenylenediamine peroxidase activity Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.1 PGHS
-
 Homo sapiens
1.14.99.1 prostaglandin H synthase
-
 Homo sapiens