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Literature summary extracted from
- Properties of the cysteine residues and iron-sulfur cluster of the assimilatory 5'-adenylyl sulfate reductase from Pseudomonas aeruginosa (2004), Biochemistry, 43, 13478-13486.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.99.2 | expression in Escherichia coli of a recombinant His-tagged protein and several mutants | Pseudomonas aeruginosa |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.99.2 | C139S | inactive mutant with no iron present | Pseudomonas aeruginosa |
| 1.8.99.2 | C140S | catalytic iron content is retained, but the mutant is inactive | Pseudomonas aeruginosa |
| 1.8.99.2 | C228S | inactive mutant with no iron present | Pseudomonas aeruginosa |
| 1.8.99.2 | C231S | inactive mutant with no iron present | Pseudomonas aeruginosa |
| 1.8.99.2 | C256S | catalytic iron content is retained, but the mutant is inactive | Pseudomonas aeruginosa |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.2 | Iron | Cys139, Cys228 and Cys231 act as ligands to a [3Fe-4S] cluster | Pseudomonas aeruginosa |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.2 | 35000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
| 1.8.99.2 | 35430 | - |
mass spectrometry | Pseudomonas aeruginosa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.99.2 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.99.2 | purification of a recombinant His-tagged protein and several mutants by Ni2+ affinity chromatography | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.99.2 | adenylyl sulfate + reduced thioredoxin | - |
Pseudomonas aeruginosa | AMP + sulfite + oxidized thioredoxin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.99.2 | monomer | 1 * 35000, SDS-PAGE, a homodimer can be formed in solution depending on the redox potential | Pseudomonas aeruginosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.99.2 | APS reductase | - |
Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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