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Literature summary extracted from

  • Wijma, H.J.; Canters, G.W.; de Vries, S.; Verbeet, M.P.
    Bidirectional catalysis by copper-containing nitrite reductase (2004), Biochemistry, 43, 10467-10474.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.1
-
 Alcaligenes faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.1 0.036
-
 nitrite 25°C, pH 7.0 Alcaligenes faecalis
1.7.2.1 0.049
-
 nitrite 25°C, pH 6.0 Alcaligenes faecalis
1.7.2.1 0.053
-
 nitrite 25°C, pH 6.5 Alcaligenes faecalis
1.7.2.1 0.067
-
 reduced pseudoazurin 25°C, pH 6.0 Alcaligenes faecalis
1.7.2.1 0.068
-
 nitrite 25°C, pH 7.5 Alcaligenes faecalis
1.7.2.1 0.077
-
 reduced pseudoazurin 25°C, pH 6.5 Alcaligenes faecalis
1.7.2.1 0.085
-
 reduced pseudoazurin 25°C, pH 8.0 Alcaligenes faecalis
1.7.2.1 0.1
-
 reduced pseudoazurin 25°C, pH 7.0 Alcaligenes faecalis
1.7.2.1 0.16
-
 reduced pseudoazurin 25°C, pH 7.5 Alcaligenes faecalis
1.7.2.1 0.22
-
 nitrite 25°C, pH 8.0 Alcaligenes faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.1 copper copper containing nitrite reductase Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.1 nitrite + reduced pseudoazurin Alcaligenes faecalis
-
 NO + oxidized pseudoazurin
-
 r
1.7.2.1 nitrite + reduced pseudoazurin Alcaligenes faecalis S-6
-
 NO + oxidized pseudoazurin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Alcaligenes faecalis
-
 S-6
-
1.7.2.1 Alcaligenes faecalis S-6
-
 S-6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.1 recombinant enzyme Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 nitrite + reduced pseudoazurin
-
 Alcaligenes faecalis NO + oxidized pseudoazurin
-
 r
1.7.2.1 nitrite + reduced pseudoazurin
-
 Alcaligenes faecalis S-6 NO + oxidized pseudoazurin
-
 r
1.7.2.1 nitrite + reduced pseudoazurin + H+
-
 Alcaligenes faecalis nitric oxide + oxidized pseudoazurin + H2O
-
 r
1.7.2.1 nitrite + reduced pseudoazurin + H+
-
 Alcaligenes faecalis S-6 nitric oxide + oxidized pseudoazurin + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.7.2.1 AfNiR
-
 Alcaligenes faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.1 38
-
 NO 25°C, pH 6.5 Alcaligenes faecalis
1.7.2.1 64
-
 NO 25°C, pH 7.0 Alcaligenes faecalis
1.7.2.1 71
-
 nitrite 25°C, pH 8.0 Alcaligenes faecalis
1.7.2.1 109
-
 NO 25°C, pH 7.5 Alcaligenes faecalis
1.7.2.1 125
-
 NO 25°C, pH 8.0 Alcaligenes faecalis
1.7.2.1 219
-
 nitrite 25°C, pH 7.5 Alcaligenes faecalis
1.7.2.1 392
-
 nitrite 25°C, pH 7.0 Alcaligenes faecalis
1.7.2.1 1046
-
 nitrite 25°C, pH 6.5 Alcaligenes faecalis
1.7.2.1 1478
-
 nitrite 25°C, pH 6.0 Alcaligenes faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.1 6
-
-
 Alcaligenes faecalis