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Literature summary extracted from

  • Reijnierse, G.L.A.; Veldstra, H.; van den Berg, C.J.
    Radioassay of acetyl-CoA synthetase, propionyl-CoA synthetase and butyryl-CoA synthetase in brain (1976), Anal. Biochem., 72, 614-622.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.2.1.17 analysis rapid radiochemical assay method with high sensitivity and specificity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.2 dithiothreitol
-
 Rattus norvegicus
6.2.1.2 GSH
-
 Rattus norvegicus
6.2.1.17 dithiothreitol 5 or 10 mM Rattus norvegicus
6.2.1.17 GSH 5 or 20 mM Rattus norvegicus
6.2.1.17 MgCl2 above 10 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.2 4.2
-
 Butyrate
-
 Rattus norvegicus
6.2.1.17 2.7
-
 propanoate
-
 Rattus norvegicus
6.2.1.17 4.6
-
 Butyrate
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.17 Mg2+ optimal concentration: 4.0 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.2 Rattus norvegicus
-
-
-
6.2.1.17 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.2 brain
-
 Rattus norvegicus
-
6.2.1.17 brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.2 ATP + butyrate + CoA
-
 Rattus norvegicus AMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.17 ATP + butyrate + CoA
-
 Rattus norvegicus AMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.17 ATP + propanoate + CoA
-
 Rattus norvegicus AMP + diphosphate + propanoyl-CoA
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.17 25 37 at 25°C the activity is 55% of that at 37°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.2 7.1
-
-
 Rattus norvegicus
6.2.1.17 8
-
-
 Rattus norvegicus