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Literature summary extracted from

  • Segraves, E.N.; Holman, T.R.
    Kinetic investigations of the rate-limiting step in human 12- and 15-lipoxygenase (2003), Biochemistry, 42, 5236-5243.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31
-
 Homo sapiens
1.13.11.33
-
 Homo sapiens

General Stability

EC Number General Stability Organism
1.13.11.31 severe autoinactivation is observed at high substrate concentrations if the substrate is not HPLC-purified and stored at -80°C Homo sapiens
1.13.11.33 severe autoinactivation is observed at high substrate concentrations if the substrate is not HPLC-purified and stored at -80°C Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.31 7
-
 O2
-
 Homo sapiens
1.13.11.33 4.2
-
 O2
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Homo sapiens
-
-
-
1.13.11.33 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31
-
 Homo sapiens
1.13.11.33
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 blood platelet
-
 Homo sapiens
-
1.13.11.33 reticulocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonate + O2
-
 Homo sapiens (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?
1.13.11.31 linoleic acid + O2 4% of the activity with arachidonate Homo sapiens ?
-
 ?
1.13.11.33 arachidonate + O2
-
 Homo sapiens (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?
1.13.11.33 linoleic acid + O2 4% of the activity with arachidonate Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 12-hLO
-
 Homo sapiens
1.13.11.33 15-hLO
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.31 2.5
-
 arachidonate pH 7.5, 15°C Homo sapiens
1.13.11.31 13.1
-
 O2
-
 Homo sapiens
1.13.11.31 17.8
-
 arachidonate pH 7.5, 30°C Homo sapiens
1.13.11.31 46
-
 arachidonate pH 7.5, 45°C Homo sapiens
1.13.11.33 5.2
-
 O2
-
 Homo sapiens
1.13.11.33 10.2
-
 linoleic acid pH 7.5, 20°C Homo sapiens
1.13.11.33 17.8
-
 linoleic acid pH 7.5, 30°C Homo sapiens