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Literature summary extracted from

  • van Boxel, G.I.; Quirk, P.G.; Cotton, N.P.; White, S.A.; Jackson, J.B.
    Glutamine 132 in the NAD(H)-binding component of proton-translocating transhydrogenase tethers the nucleotides before hydride transfer (2003), Biochemistry, 42, 1217-1226.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.1 domains expressed separately in Escherichia coli Rhodospirillum rubrum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.1.1.1 (dI.Q132N)2dIII trimer Rhodospirillum rubrum

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.1 E155W dIII domain Rhodospirillum rubrum
7.1.1.1 Q132N dI domain, no cyclic transhydrogenation activity in mixtures of domain dIII with the dI mutant, mutation has little effect on the NADH binding affinity Rhodospirillum rubrum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.1 membrane
-
 Rhodospirillum rubrum 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.1 NADH + NADP+ Rhodospirillum rubrum links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane NAD+ + NADPH
-
 r
7.1.1.1 NADPH + oxidized 3-acetylpyridine adenine dinucleotide Rhodospirillum rubrum
-
 NADP+ + reduced 3-acetylpyridine adenine dinucleotide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.1 Rhodospirillum rubrum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.1 recombinant proteins Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.1 NADH + NADP+
-
 Rhodospirillum rubrum NAD+ + NADPH
-
 r
7.1.1.1 NADH + NADP+ links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane Rhodospirillum rubrum NAD+ + NADPH
-
 r
7.1.1.1 NADPH + oxidized 3-acetylpyridine adenine dinucleotide
-
 Rhodospirillum rubrum NADP+ + reduced 3-acetylpyridine adenine dinucleotide
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.1 trimer mixture of isolated domains dI and dIII forms dI2dIII trimers, formation of trimers also with Q132N mutant dI domain Rhodospirillum rubrum

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.1 NAD(P)+
-
 Rhodospirillum rubrum
7.1.1.1 NAD(P)H
-
 Rhodospirillum rubrum