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Literature summary extracted from

  • Aitken, S.M.; Kim, D.H.; Kirsch, J.F.
    Escherichia coli cystathionine gamma-synthase does not obey ping-pong kinetics. Novel continuous assays for the elimination and substitution reactions (2003), Biochemistry, 42, 11297-11306.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.48 cloned from strain JM103, expression in strain DH10B Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.48 additional information
-
 additional information detailed kinetics of elimination and replacement reaction, overview Escherichia coli
2.5.1.48 0.1
-
 L-cysteine pH 7.8, 25°C, replacement reaction, ordered mechanism Escherichia coli
2.5.1.48 0.11
-
 L-cysteine pH 7.8, 25°C, replacement reaction, modified ping-pong mechanism Escherichia coli
2.5.1.48 1.2
-
 O-succinyl-L-homoserine pH 7.8, 25°C, replacement reaction, ordered mechanism Escherichia coli
2.5.1.48 1.3
-
 O-succinyl-L-homoserine pH 7.8, 25°C, elimination reaction, modified ping-pong mechanism Escherichia coli
2.5.1.48 2.5
-
 O-succinyl-L-homoserine pH 7.8, 25°C, replacement reaction, modified ping-pong mechanism Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.48 O-succinyl-L-homoserine + L-cysteine Escherichia coli first step in bacterial transsulfuration pathway L-cystathionine + succinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.48 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.48 O4-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate enzyme performs an elimination or a replacement reaction, reaction mechanism, formation of an aldimine intermediate, overview Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.48 additional information enzyme performs an elimination or a replacement reaction Escherichia coli ?
-
 ?
2.5.1.48 O-succinyl-L-homoserine elimination reaction Escherichia coli succinate + pyruvate + NH3
-
 ?
2.5.1.48 O-succinyl-L-homoserine + L-cysteine first step in bacterial transsulfuration pathway Escherichia coli L-cystathionine + succinate
-
 ?
2.5.1.48 O-succinyl-L-homoserine + L-cysteine replacement reaction Escherichia coli L-cystathionine + succinate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.48 CGS
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.48 25
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.48 1.8
-
 O-succinyl-L-homoserine pH 7.8, 25°C, elimination reaction, modified ping-pong mechanism Escherichia coli
2.5.1.48 117
-
 L-cysteine pH 7.8, 25°C, replacement reaction, ordered mechanism Escherichia coli
2.5.1.48 117
-
 O-succinyl-L-homoserine pH 7.8, 25°C, replacement reaction, ordered mechanism Escherichia coli
2.5.1.48 121
-
 L-cysteine pH 7.8, 25°C, replacement reaction, modified ping-pong mechanism Escherichia coli
2.5.1.48 121
-
 O-succinyl-L-homoserine pH 7.8, 25°C, replacement reaction, modified ping-pong mechanism Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.48 7.4
-
 elimination reaction Escherichia coli
2.5.1.48 7.8
-
 replacement reaction Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.48 pyridoxal 5'-phosphate dependent on Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.48 additional information
-
 additional information
-
 Escherichia coli