EC Number | Cloned (Comment) | Organism |
---|---|---|
1.6.2.2 | mutant enzymes expressed in Escherichia coli BL21(DE3)-RIL as His-tag fusion proteins | Rattus norvegicus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.6.2.2 | sitting drop method, complete data set collected for the D239T mutant enzyme | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.6.2.2 | D239E | decreased activity with NADH and NADPH | Rattus norvegicus |
1.6.2.2 | D239S | significantly increased activity with NADPH | Rattus norvegicus |
1.6.2.2 | D239S/F251R | specific for NADPH | Rattus norvegicus |
1.6.2.2 | D239S/F251Y | bispecific for NADH and NADPH | Rattus norvegicus |
1.6.2.2 | D239T | specific for NADPH, 11fold preference for NADPH over NADH | Rattus norvegicus |
1.6.2.2 | D239T/F251R | specific for NADPH | Rattus norvegicus |
1.6.2.2 | F251R | minor effects on activity | Rattus norvegicus |
1.6.2.2 | F251Y | minor effects on activity | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.2 | 0.003 | - |
NADH | F251Y mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.005 | - |
NADH | D239S/F251Y mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.006 | - |
NADH | D239E mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.006 | - |
NADH | wild type enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.009 | - |
NADPH | D239T mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.012 | - |
NADH | F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.017 | - |
NADH | D239S mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.022 | - |
NADPH | D239S/F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.025 | - |
NADPH | D239S/F251Y mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.044 | - |
NADPH | D239T/F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.094 | - |
NADPH | D239E mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.119 | - |
NADH | D239T mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.138 | - |
NADPH | F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.224 | - |
NADH | D239S/F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.268 | - |
NADPH | D239S mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.512 | - |
NADH | D239T/F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.617 | - |
NADPH | F251Y mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 0.924 | - |
NADPH | wild type enzyme, pH 7.0, 25°C | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.2 | 2 ferricytochrome b5 + NADH | Rattus norvegicus | involved in the synthesis of fatty acids and cholesterol, and in the oxidation of xenobiotics | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.2.2 | Rattus norvegicus | P20070 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.2.2 | recombinant enzymes | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.2 | 2 ferricyanide + NADPH | - |
Rattus norvegicus | 2 ferrocyanide + NADP+ + H+ | - |
? | |
1.6.2.2 | 2 ferricytochrome b5 + NADH | - |
Rattus norvegicus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
1.6.2.2 | 2 ferricytochrome b5 + NADH | involved in the synthesis of fatty acids and cholesterol, and in the oxidation of xenobiotics | Rattus norvegicus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.2 | 5.2 | - |
NADPH | D239E mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 33 | - |
NADPH | wild type enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 50 | - |
NADPH | F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 50 | - |
NADPH | F251Y mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 200 | - |
NADPH | D239S/F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 217 | - |
NADPH | D239S mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 250 | - |
NADH | D239S/F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 267 | - |
NADPH | D239T mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 333 | - |
NADH | D239T mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 367 | - |
NADH | F251Y mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 417 | - |
NADPH | D239S/F251Y mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 433 | - |
NADH | D239S mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 467 | - |
NADH | D239S/F251Y mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 500 | - |
NADH | F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 517 | - |
NADH | D239E mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 550 | - |
NADPH | D239T/F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 717 | - |
NADH | D239T/F251R mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
1.6.2.2 | 800 | - |
NADH | wild type enzyme, pH 7.0, 25°C | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.2 | FAD | - |
Rattus norvegicus | |
1.6.2.2 | NADH | native enzyme shows 3700fold preference for NADH over NADPH | Rattus norvegicus | |
1.6.2.2 | NADPH | preferred cofactor for some engineered enzymes | Rattus norvegicus |