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Literature summary extracted from

  • Wolan, D.W.; Greasley, S.E.; Wall, M.J.; Benkovic, S.J.; Wilson, I.A.
    Structure of avian AICAR transformylase with a multisubstrate adduct inhibitor beta-DADF identifies the folate binding site (2003), Biochemistry, 42, 10904-10914.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.3 expressed in Escherichia coli as His-tag fusion protein Gallus gallus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.3 recombinant His-tag fusion protein crystallized by sitting drop vapor diffusion method in complex with its inhibitor beta-DADF Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.3 beta-DADF IC50 125 nM Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide Gallus gallus penultimate step of the purine de novo synthesis pathway tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.3 Gallus gallus P31335
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.3
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide
-
 Gallus gallus tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
 ?
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamide penultimate step of the purine de novo synthesis pathway Gallus gallus tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.3 dimer crystal structure analysis, monomer contains two functionally independent domains transformylase and cyclohydrolase activities Gallus gallus

Synonyms

EC Number Synonyms Comment Organism
2.1.2.3 AICAR transformylase/inosine monophosphate cyclohydrolase bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.3 10-formyltetrahydrofolate
-
 Gallus gallus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.2.3 0.000125
-
 IC50 125 nM Gallus gallus beta-DADF