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Literature summary extracted from

  • Pang, S.S.; Duggleby, R.G.
    Expression, purification, characterization, and reconstitution of the large and small subunits of yeast acetohydroxyacid synthase (1999), Biochemistry, 38, 5222-5231.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 L-valine not inhibitory for catalytic subunit alone, inhibitory for catalytic subunit plus small subunit Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Saccharomyces cerevisiae
-
 expression of both small and catalytic subunit in Escherichia coli
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.2.1.6 reconstitution of enzyme from small and catalytic subunit each overexpressed in Escherichia coli and purified. Small subunit protein stimulates catalytic subunit up to 7fold and confers upon it sensitivity to inhibition by valine Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 6.8
-
 catalytic subunit, pH 7.0, 30°C Saccharomyces cerevisiae
2.2.1.6 49
-
 catalytic subunit plus small subunit, pH 7.0, 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 pyruvate
-
 Saccharomyces cerevisiae 2-acetolactate + CO2
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.2.1.6 0.16
-
 L-valine catalytic subunit plus small subunit, pH 7.0, 30°C Saccharomyces cerevisiae