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Literature summary extracted from

  • Wu, J.; Sheflyan, G.Y.; Woodard, R.W.
    Bacillus subtilis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase revisited: resolution of two long-standing enigmas (2005), Biochem. J., 390, 583-590.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 chorismate 1 mM, 3% residual activity Bacillus subtilis
2.5.1.54 dipicolinic acid inactivation, reactivation by divalent cations Bacillus subtilis
2.5.1.54 L-phenylalanine 1 mM, 3% residual activity Bacillus subtilis
2.5.1.54 L-tryptophan 1 mM, 3% residual activity Bacillus subtilis
2.5.1.54 L-tyrosine 1 mM, 3% residual activity Bacillus subtilis
2.5.1.54 additional information no inhibitory: EDTA Bacillus subtilis
2.5.1.54 prephenate 1 mM, 3% residual activity Bacillus subtilis
2.5.1.54 shikimate 1 mM, 3% residual activity Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.139
-
 phosphoenolpyruvate pH 7.5, 37°C Bacillus subtilis
2.5.1.54 1.76
-
 D-erythrose 4-phosphate pH 7.5, 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Iron
-
 Bacillus subtilis
2.5.1.54 Zn2+
-
 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 156000
-
 gel filtration Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Bacillus subtilis
-
 Marburg strain and strain 168, bifunctional enzyme with chorismate mutase activity in addition to enzymic activity
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.5.1.54 reactivation of dipicolinic acid-inactivated enzyme by divalent cations Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Bacillus subtilis 3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 tetramer 4 * 4000, SDS-PAGE Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 4.6
-
 phosphoenolpyruvate pH 7.5, 37°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 9
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.54 8.5 9.5 more than 85% of maximum activity Bacillus subtilis