Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ahmed, H.; Ettema, T.J.; Tjaden, B.; Geerling, A.C.; van der Oost, J.; Siebers, B.
    The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation (2005), Biochem. J., 390, 529-540.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.45 gene kdgK as part of the ED gene cluster, expresssion in Escherichia coli Saccharolobus solfataricus
2.7.1.45 gene kdgK as part of the ED gene cluster, expresssion in Escherichia coli as soluble proein at low rate Thermoproteus tenax

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.45 additional information reconstruction of the Entner-Doudoroff pathway with recombinantly produced enzymes of Thermoproteus tenax and Solfolobus solfataricus, overview Saccharolobus solfataricus
2.7.1.45 additional information reconstruction of the Entner-Doudoroff pathway with recombinantly produced enzymes of Thermoproteus tenax and Solfolobus solfataricus, overview Thermoproteus tenax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.45 0.178
-
 2-dehydro-3-deoxy-D-gluconate pH 7.0, 70°C, recombinant enzyme Thermoproteus tenax

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.45 Mg2+ absolutely dependent on Saccharolobus solfataricus
2.7.1.45 Mg2+ absolutely dependent on Thermoproteus tenax

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.45 32000
-
 x * 32000, recombinant enzyme, SDS-PAGE, x * 33308, sequence calculation Thermoproteus tenax
2.7.1.45 33308
-
 x * 32000, recombinant enzyme, SDS-PAGE, x * 33308, sequence calculation Thermoproteus tenax

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.55 2-dehydro-3-deoxy-D-galactonate Thermoproteus tenax key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate Saccharolobus solfataricus key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate Saccharolobus solfataricus P2 key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate 6-phosphate Thermoproteus tenax key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate 6-phosphate Saccharolobus solfataricus key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate 6-phosphate Saccharolobus solfataricus P2 key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate Thermoproteus tenax key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus P2 key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate 6-phosphate Thermoproteus tenax key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate 6-phosphate Saccharolobus solfataricus key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate 6-phosphate Saccharolobus solfataricus P2 key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.2.1.140 D-galactonate Saccharolobus solfataricus the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.140 D-gluconate Saccharolobus solfataricus the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.55 Saccharolobus solfataricus Q97U28
-
-
4.1.2.55 Saccharolobus solfataricus P2 Q97U28
-
-
4.1.2.55 Thermoproteus tenax
-
-
-
4.2.1.140 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.45 recombinant enzyme from Escherichia coli, by heat precipitation and gel filtration Thermoproteus tenax

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.45 additional information the archaeon grows optimally at 80-85°C and pH 2-4 Saccharolobus solfataricus
-
2.7.1.45 additional information the archaeon grows optimally at about 90°C and pH 5.0 Thermoproteus tenax
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [”mol/min/mg] Specific Activity Maximum [”mol/min/mg] Comment Organism
2.7.1.45 additional information
-
-
 Saccharolobus solfataricus
2.7.1.45 additional information
-
-
 Thermoproteus tenax
2.7.1.45 0.104
-
 recombinant cell extract after heat precipitation step Saccharolobus solfataricus
2.7.1.45 41.3
-
 purified recombinant enzyme Thermoproteus tenax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.55 2-dehydro-3-deoxy-D-galactonate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Thermoproteus tenax pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Saccharolobus solfataricus P2 pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate 6-phosphate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Thermoproteus tenax pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate 6-phosphate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Saccharolobus solfataricus pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate 6-phosphate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Saccharolobus solfataricus P2 pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Thermoproteus tenax pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Saccharolobus solfataricus P2 pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate 6-phosphate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Thermoproteus tenax pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate 6-phosphate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Saccharolobus solfataricus pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate 6-phosphate key enzyme in both the non- and the semi-phosphorylative Entner–Doudoroff pathway Saccharolobus solfataricus P2 pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 pyruvate + D-glyceraldehyde
-
 Thermoproteus tenax 2-dehydro-3-deoxy-D-gluconate
-
 r
4.1.2.55 pyruvate + D-glyceraldehyde
-
 Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate
-
 r
4.1.2.55 pyruvate + D-glyceraldehyde
-
 Saccharolobus solfataricus P2 2-dehydro-3-deoxy-D-gluconate
-
 r
4.1.2.55 pyruvate + D-glyceraldehyde 3-phosphate
-
 Thermoproteus tenax 2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
 r
4.1.2.55 pyruvate + D-glyceraldehyde 3-phosphate
-
 Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
 r
4.2.1.140 D-galactonate the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway Saccharolobus solfataricus 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.140 D-galactonate crude extract of cells grown on D-glucose shows galactonate dehydratase activity and gluconate dehydratase activity Saccharolobus solfataricus 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.140 D-gluconate the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.140 D-gluconate crude extract of cells grown on D-glucose shows galactonate dehydratase activity and gluconate dehydratase activity Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.45 ? x * 32000, recombinant enzyme, SDS-PAGE, x * 33308, sequence calculation Thermoproteus tenax

Synonyms

EC Number Synonyms Comment Organism
4.2.1.140 Sso3198
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.45 additional information
-
 the archaeon grows optimally at 80-85°C and pH 2-4 Saccharolobus solfataricus
2.7.1.45 additional information
-
 the archaeon grows optimally at about 90°C and pH 5.0 Thermoproteus tenax
2.7.1.45 60
-
 assay at Saccharolobus solfataricus
2.7.1.45 70
-
 assay at Thermoproteus tenax

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.45 additional information
-
 the archaeon grows optimally at 80-85°C and pH 2-4 Saccharolobus solfataricus
2.7.1.45 additional information
-
 the archaeon grows optimally at about 90°C and pH 5.0 Thermoproteus tenax
2.7.1.45 7
-
 assay at Saccharolobus solfataricus
2.7.1.45 7
-
 assay at Thermoproteus tenax

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.45 ATP absolutely dependent on Saccharolobus solfataricus
2.7.1.45 ATP absolutely dependent on Thermoproteus tenax

General Information

EC Number General Information Comment Organism
4.2.1.140 physiological function the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the Entner–Doudoroff pathway Saccharolobus solfataricus