EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.26 | soluble active form of human XT-I is expressed in High Five insect cells, Trichoplusia ni | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.2.26 | C257A | 2.3fold decrease in the ratio of Vmax to KM-value as compared to wild-type enzyme | Homo sapiens |
2.4.2.26 | C285A | 5.4fold decrease in the ratio of Vmax to KM-value as compared to wild-type enzyme | Homo sapiens |
2.4.2.26 | C301A | 2.3fold decrease in the ratio of Vmax to KM-value as compared to wild-type enzyme | Homo sapiens |
2.4.2.26 | C471A | complete loss of catalytic activity. N-phenylmaleimide treatment shows no effect on wild-type XT-I but strongly inactivates the cysteine mutant | Homo sapiens |
2.4.2.26 | C542A | 2.9fold decrease in the ratio of Vmax to KM-value as compared to wild-type enzyme | Homo sapiens |
2.4.2.26 | C561A | UDP inhibition is significantly reduced | Homo sapiens |
2.4.2.26 | C563A | 2fold decrease in the ratio of Vmax to KM-value as compared to wild-type enzyme | Homo sapiens |
2.4.2.26 | C574A | complete loss of catalytic activity. N-phenylmaleimide treatment shows no effect on wild-type XT-I but strongly inactivates the cysteine mutant | Homo sapiens |
2.4.2.26 | C675A | 1.5fold increase in the ratio of Vmax to KM-value as compared to wild-type enzyme | Homo sapiens |
2.4.2.26 | C902A | 1.5fold increase in the ratio of Vmax to KM-value as compared to wild-type enzyme | Homo sapiens |
2.4.2.26 | C927A | 1.4fold decrease in the ratio of Vmax to KM-value as compared to wild-type enzyme | Homo sapiens |
2.4.2.26 | C933A | 1.36fold increase in the ratio of Vmax to KM-value as compared to wild-type enzyme | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.26 | N-Phenylmaleimide | treatment shows no effect on wild-type XT-I but strongly inactivaets the cysteine mutants C461A and C574A | Homo sapiens | |
2.4.2.26 | UDP | inhibition on the XT-I activity of C561A mutant enzyme is significantly reduced compared with all other tested cysteine mutants | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.26 | 0.001 | - |
bikunin | pH 6.5, 37°C, wild-type enzyme | Homo sapiens | |
2.4.2.26 | 0.001 | - |
bikunin | pH 6.5, 37°C, mutant enzyme C933A | Homo sapiens | |
2.4.2.26 | 0.0012 | - |
bikunin | pH 6.5, 37°C, mutant enzyme C920A | Homo sapiens | |
2.4.2.26 | 0.0014 | - |
bikunin | pH 6.5, 37°C, mutant enzyme C301A | Homo sapiens | |
2.4.2.26 | 0.0014 | - |
bikunin | pH 6.5, 37°C, mutant enzyme C675A | Homo sapiens | |
2.4.2.26 | 0.0016 | - |
bikunin | pH 6.5, 37°C, mutant enzyme C542A | Homo sapiens | |
2.4.2.26 | 0.002 | - |
bikunin | pH 6.5, 37°C, mutant enzyme C927A | Homo sapiens | |
2.4.2.26 | 0.0028 | - |
bikunin | pH 6.5, 37°C, mutant enzyme C285A | Homo sapiens | |
2.4.2.26 | 0.0053 | - |
bikunin | pH 6.5, 37°C, mutant enzyme C257A | Homo sapiens | |
2.4.2.26 | 0.0119 | - |
bikunin | pH 6.5, 37°C, mutant enzyme C563A | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.26 | 90000 | - |
1 * 90000, SDS-PAGE under reducing conditions | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.26 | Homo sapiens | Q86Y38 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.26 | UDP-D-xylose + bikunin | - |
Homo sapiens | UDP + D-xylosyl-bikunin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.26 | monomer | 1 * 90000, SDS-PAGE under reducing conditions | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.26 | XT-I | - |
Homo sapiens |
2.4.2.26 | xylosyltransferase I | - |
Homo sapiens |