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Literature summary extracted from

  • Sundaram, A.K.; Pitts, L.; Muhammad, K.; Wu, J.; Betenbaugh, M.; Woodard, R.W.; Vann, W.F.
    Characterization of N-acetylneuraminic acid synthase isoenzyme 1 from Campylobacter jejuni (2004), Biochem. J., 383, 83-89.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.56 EDTA 10 mM, complete inhibition Campylobacter jejuni
2.5.1.56 Zn2+ 0.1 mM, 68% of initial activity Campylobacter jejuni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.56 5.5
-
N-propanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
2.5.1.56 7.3
-
phosphoenolpyruvate pH 8.0, 37°C Campylobacter jejuni
2.5.1.56 15
-
N-butanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
2.5.1.56 17.6
-
N-acetyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
2.5.1.56 31.1
-
N-pentanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.56 Co2+ 0.1 mM, 122% of initial activity Campylobacter jejuni
2.5.1.56 Mg2+ 0.1 mM, 101% of initial activity Campylobacter jejuni
2.5.1.56 Mn2+ 0.1 mM, 150% of initial activity Campylobacter jejuni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.56 43300
-
x * 43300, His-tagged recombinant enzyme, MALDI-TOF MS Campylobacter jejuni

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.56 Campylobacter jejuni
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.56 1.018
-
pH 8.0, 37°C Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.56 additional information enzyme catalyzes the condensation of the si face of phosphoenolpyruvate with the aldehyde sugar Campylobacter jejuni ?
-
?
2.5.1.56 phosphoenolpyruvate + N-acetyl-D-mannosamine + H2O
-
Campylobacter jejuni phosphate + N-acetylneuraminate
-
?
2.5.1.56 phosphoenolpyruvate + N-butanoyl-D-mannosamine + H2O
-
Campylobacter jejuni phosphate + N-butanoylneuraminate
-
?
2.5.1.56 phosphoenolpyruvate + N-pentanoyl-D-mannosamine + H2O
-
Campylobacter jejuni phosphate + N-pentanoylneuraminate
-
?
2.5.1.56 phosphoenolpyruvate + N-propanoyl-D-mannosamine + H2O
-
Campylobacter jejuni phosphate + N-propanoylneuraminate
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.56 ? x * 43300, His-tagged recombinant enzyme, MALDI-TOF MS Campylobacter jejuni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.56 0.8
-
N-pentanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
2.5.1.56 3.5
-
N-butanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
2.5.1.56 11.8
-
N-propanoyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni
2.5.1.56 19
-
phosphoenolpyruvate pH 8.0, 37°C Campylobacter jejuni
2.5.1.56 19.9
-
N-acetyl-D-mannosamine pH 8.0, 37°C Campylobacter jejuni