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Literature summary extracted from

  • Wu, J.; Patel, M.A.; Sundaram, A.K.; Woodard, R.W.
    Functional and biochemical characterization of a recombinant Arabidopsis thaliana 3-deoxy-D-manno-octulosonate 8-phosphate synthase (2004), Biochem. J., 381, 185-193.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.55 cloned into a T7-driven expression vector, overexpression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.55 0.0036
-
 phosphoenolpyruvate 37°C Arabidopsis thaliana
2.5.1.55 0.0038
-
 D-arabinose 5-phosphate 37°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.55 additional information non-metallo enzyme, no metal requirement Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.55 31519
-
 2 * 31519, MALDI-MS Arabidopsis thaliana
2.5.1.55 76000
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.55 recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate
-
 Arabidopsis thaliana 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.55 dimer 2 * 31519, MALDI-MS Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.5.1.55 3-deoxy-D-manno-octulosonate 8-phosphate synthase
-
 Arabidopsis thaliana
2.5.1.55 KDOPS
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.55 65
-
-
 Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.55 45 70 45°C: about 50% of maximal activity, 70°C: about 55% of maximal activity Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.55 40
-
 30 min, in absence of any substrate or in presence of D-arabinose 5-phosphate, 30% loss of activity, no inactivation in presence of phosphoenolpyruvate Arabidopsis thaliana
2.5.1.55 50
-
 30 min, in absence of any substrate or in presence of D-arabinose 5-phosphate, 30% loss of activity, no inactivation in presence of phosphoenolpyruvate Arabidopsis thaliana
2.5.1.55 60
-
 20% incrase in activity after 7 min Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.55 5.9
-
 phosphoenolpyruvate 37°C Arabidopsis thaliana
2.5.1.55 5.9
-
 D-arabinose 5-phosphate 37°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.55 7.5
-
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.55 6.5 9.5 broad peak between pH 6.5 and 9.5 showing 90% of maximal activity Arabidopsis thaliana