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Literature summary extracted from
- Plant sterol biosynthesis: identification of two distinct families of sterol 4alpha-methyl oxidases (2004), Biochem. J., 378, 889-898.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.18.10 | DNA and amino acid sequence determination and analysis | Zea mays |
| 1.14.18.10 | DNA and amino acid sequence determination and analysis | Nicotiana tabacum |
| 1.14.18.10 | DNA and amino acid sequence determination and analysis, expression in enzyme-lacking Saccharomyces cerevisiae strain erg25 | Arabidopsis thaliana |
| 1.14.18.10 | DNA and amino acid sequence determination and analysis, in vitro transcription | Nicotiana benthamiana |
| 1.14.18.11 | DNA and amino acid sequence determination and analysis | Nicotiana tabacum |
| 1.14.18.11 | DNA and amino acid sequence determination and analysis, expression in enzyme-lacking Saccharomyces cerevisiae strain erg25 | Arabidopsis thaliana |
| 1.14.18.11 | DNA and amino acid sequence determination and analysis, in vitro transcription | Nicotiana benthamiana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.18.10 | additional information | virus-iduced gene silencing approach for reduction of enzyme expression, sterol analysis of transgenic plants | Nicotiana benthamiana |
| 1.14.18.11 | additional information | virus-iduced gene silencing approach for reduction of enzyme expression, sterol analysis of transgenic plants | Nicotiana benthamiana |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.10 | Iron | non-heme iron | Zea mays |  |
| 1.14.18.10 | Iron | non-heme iron | Arabidopsis thaliana | |
| 1.14.18.10 | Iron | non-heme iron | Nicotiana benthamiana | |
| 1.14.18.10 | Iron | non-heme iron | Nicotiana tabacum | |
| 1.14.18.11 | Iron | non-heme iron | Arabidopsis thaliana | |
| 1.14.18.11 | Iron | non-heme iron | Nicotiana benthamiana | |
| 1.14.18.11 | Iron | non-heme iron | Nicotiana tabacum | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.10 | additional information | Zea mays | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | ? | - |
? | |
| 1.14.18.10 | additional information | Arabidopsis thaliana | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | ? | - |
? | |
| 1.14.18.10 | additional information | Nicotiana tabacum | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | ? | - |
? | |
| 1.14.18.10 | additional information | Nicotiana benthamiana | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview | ? | - |
? | |
| 1.14.18.10 | additional information | Zea mays SMO1 | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | ? | - |
? | |
| 1.14.18.11 | additional information | Arabidopsis thaliana | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | ? | - |
? | |
| 1.14.18.11 | additional information | Nicotiana tabacum | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | ? | - |
? | |
| 1.14.18.11 | additional information | Nicotiana benthamiana | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.10 | Arabidopsis thaliana | - |
- |
- |
| 1.14.18.10 | Arabidopsis thaliana | F4JLZ6 | isozyme SMO1-3; 2 distinct enzyme families exist with 3 and 2 isozymes belonging to them, respectively | - |
| 1.14.18.10 | Arabidopsis thaliana | Q1EC69 | isozyme SMO1-2; 2 distinct enzyme families exist with 3 and 2 isozymes belonging to them, respectively | - |
| 1.14.18.10 | Arabidopsis thaliana | Q8L7W5 | isozyme SMO1-1; 2 distinct enzyme families exist with 3 and 2 isozymes belonging to them, respectively | - |
| 1.14.18.10 | Nicotiana benthamiana | Q5J955 | isozyme SMO1; 2 isozymes SMO1 and SMO2 | - |
| 1.14.18.10 | Nicotiana tabacum | Q6YAU5 | isozyme SMO1; cv BY2, isozymes SMO1 and SMO2 | - |
| 1.14.18.10 | Zea mays | - |
SMO1 | - |
| 1.14.18.10 | Zea mays SMO1 | - |
SMO1 | - |
| 1.14.18.11 | Arabidopsis thaliana | Q8VWZ8 | isozyme SMO2-2; 2 distinct enzyme families exist with 3 and 2 isozymes belonging to them, respectively | - |
| 1.14.18.11 | Arabidopsis thaliana | Q9ZW22 | isozyme SMO2-1; 2 distinct enzyme families exist with 3 and 2 isozymes belonging to them, respectively | - |
| 1.14.18.11 | Nicotiana benthamiana | Q5J954 | isozyme SMO2; 2 isozymes SMO1 and SMO2 | - |
| 1.14.18.11 | Nicotiana tabacum | Q6YAU6 | isozyme SMO2; cv BY2, isozymes SMO1 and SMO2 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.10 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H+ + O2 | 1c | Arabidopsis thaliana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferrocytochrome + H2O | - |
? | |
| 1.14.18.10 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Zea mays | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Arabidopsis thaliana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Nicotiana benthamiana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Nicotiana tabacum | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Zea mays SMO1 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + 3 H+ + 3 O2 | overall reaction | Arabidopsis thaliana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + 4 H2O | - |
? | |
| 1.14.18.10 | 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + H+ + O2 | 1a | Arabidopsis thaliana | 4alpha-hydroxymethyl-4beta-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Zea mays | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Arabidopsis thaliana | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Nicotiana benthamiana | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Nicotiana tabacum | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Zea mays SMO1 | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 4alpha-hydroxymethyl-4beta-methyl-5alpha-cholest-7-en-3beta-ol + reduced acceptor + H+ + O2 | 1b | Arabidopsis thaliana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + acceptor + 2 H2O | - |
? | |
| 1.14.18.10 | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Zea mays | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Arabidopsis thaliana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Nicotiana benthamiana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Nicotiana tabacum | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Zea mays SMO1 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
| 1.14.18.10 | additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | Zea mays | ? | - |
? | |
| 1.14.18.10 | additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | Arabidopsis thaliana | ? | - |
? | |
| 1.14.18.10 | additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | Nicotiana tabacum | ? | - |
? | |
| 1.14.18.10 | additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview | Nicotiana benthamiana | ? | - |
? | |
| 1.14.18.10 | additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | Zea mays SMO1 | ? | - |
? | |
| 1.14.18.11 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Arabidopsis thaliana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
| 1.14.18.11 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Nicotiana benthamiana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
| 1.14.18.11 | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2 | step 3 of 3 of the overall reaction | Nicotiana tabacum | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O | - |
? | |
| 1.14.18.11 | 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Arabidopsis thaliana | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 1.14.18.11 | 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Nicotiana benthamiana | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 1.14.18.11 | 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 1 of 3 of the overall reaction | Nicotiana tabacum | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O | - |
? | |
| 1.14.18.11 | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Arabidopsis thaliana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
| 1.14.18.11 | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Nicotiana benthamiana | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
| 1.14.18.11 | 4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2 | step 2 of 3 of the overall reaction | Nicotiana tabacum | 3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O | - |
? | |
| 1.14.18.11 | additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | Arabidopsis thaliana | ? | - |
? | |
| 1.14.18.11 | additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO | Nicotiana tabacum | ? | - |
? | |
| 1.14.18.11 | additional information | conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview | Nicotiana benthamiana | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.18.10 | More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Zea mays |
| 1.14.18.10 | More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Arabidopsis thaliana |
| 1.14.18.10 | More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Nicotiana benthamiana |
| 1.14.18.10 | More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Nicotiana tabacum |
| 1.14.18.11 | More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Arabidopsis thaliana |
| 1.14.18.11 | More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Nicotiana benthamiana |
| 1.14.18.11 | More | enzyme possesses 3 His-rich motifs common for membrane-bound non-heme iron oxygenases | Nicotiana tabacum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.10 | 4alpha-methyl oxidase | - |
Zea mays |
| 1.14.18.10 | 4alpha-methyl oxidase | - |
Arabidopsis thaliana |
| 1.14.18.10 | 4alpha-methyl oxidase | - |
Nicotiana benthamiana |
| 1.14.18.10 | 4alpha-methyl oxidase | - |
Nicotiana tabacum |
| 1.14.18.10 | SMO1 | - |
Zea mays |
| 1.14.18.10 | SMO1 | - |
Nicotiana benthamiana |
| 1.14.18.10 | SMO1 | - |
Nicotiana tabacum |
| 1.14.18.10 | SMO1-1 | - |
Arabidopsis thaliana |
| 1.14.18.10 | SMO1-2 | - |
Arabidopsis thaliana |
| 1.14.18.10 | SMO1-3 | - |
Arabidopsis thaliana |
| 1.14.18.11 | 4alpha-methyl oxidase | - |
Arabidopsis thaliana |
| 1.14.18.11 | 4alpha-methyl oxidase | - |
Nicotiana benthamiana |
| 1.14.18.11 | 4alpha-methyl oxidase | - |
Nicotiana tabacum |
| 1.14.18.11 | SMO2 | - |
Nicotiana benthamiana |
| 1.14.18.11 | SMO2 | - |
Nicotiana tabacum |
| 1.14.18.11 | SMO2-1 | - |
Arabidopsis thaliana |
| 1.14.18.11 | SMO2-2 | - |
Arabidopsis thaliana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.10 | NAD(P)H | - |
Zea mays | |
| 1.14.18.10 | NAD(P)H | - |
Arabidopsis thaliana | |
| 1.14.18.10 | NAD(P)H | - |
Nicotiana benthamiana | |
| 1.14.18.10 | NAD(P)H | - |
Nicotiana tabacum | |
| 1.14.18.11 | NAD(P)H | - |
Arabidopsis thaliana | |
| 1.14.18.11 | NAD(P)H | - |
Nicotiana benthamiana | |
| 1.14.18.11 | NAD(P)H | - |
Nicotiana tabacum | |
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