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Literature summary extracted from

  • Price, N.T.; Jackson, V.N.; van der Leij, F.R.; Cameron, J.M.; Travers, M.T.; Bartelds, B.; Huijkman, N.C.; Zammit, V.A.
    Cloning and expression of the liver and muscle isoforms of ovine carnitine palmitoyltransferase 1: residues within the N-terminus of the muscle isoform influence the kinetic properties of the enzyme (2003), Biochem. J., 372, 871-879.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.21 expression in Pichia pastoris Ovis aries

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.21 additional information chimaera in which the distinctive N-terminal segmant of ovine m-BPT 1 is replaced with that from rat M-CPT 1. The ovine N-terminal segment influences the kinetics of the enzyme for both its substrates, such that the Km for palmitoyl-CoA is decreased and that for carnitine is increased for the chimera, relative to the parental enzyme M-CPT 1 from Ovis aries Ovis aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.21 malonyl-CoA IC50 for wild-type L-CPT-1 is 0.0298 mM; IC50 for wild-type M-CPT-1 is 0.010 mM Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.21 0.03
-
palmitoyl-CoA chimaera in which the distinctive N-terminal segment of ovine m-BPT 1 is replaced with that from rat M-CPT 1 Ovis aries
2.3.1.21 0.0374
-
palmitoyl-CoA wild-type L-CPT1 Ovis aries
2.3.1.21 0.0676
-
palmitoyl-CoA wild-type M-CPT1 Ovis aries
2.3.1.21 0.215
-
L-carnitine wild-type L-CPT 1 Ovis aries
2.3.1.21 0.56
-
L-carnitine wild-type M-CPT 1 Ovis aries
2.3.1.21 0.785
-
L-carnitine chimaera in which the distinctive N-terminal segment of ovine m-BPT 1 is replaced with that from rat M-CPT 1 Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.21 Ovis aries Q7YQR7
-
-
2.3.1.21 Ovis aries Q8SPI7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.21 adipose tissue
-
Ovis aries
-
2.3.1.21 brain
-
Ovis aries
-
2.3.1.21 brain high level of mRNA Ovis aries
-
2.3.1.21 heart
-
Ovis aries
-
2.3.1.21 heart high level of mRNA Ovis aries
-
2.3.1.21 kidney
-
Ovis aries
-
2.3.1.21 kidney high level of mRNA Ovis aries
-
2.3.1.21 liver
-
Ovis aries
-
2.3.1.21 liver high level of mRNA Ovis aries
-
2.3.1.21 lung
-
Ovis aries
-
2.3.1.21 mammary gland lactating, high level of mRNA Ovis aries
-
2.3.1.21 mammary gland non-lactating, high level of mRNA Ovis aries
-
2.3.1.21 additional information no activity in spleen Ovis aries
-
2.3.1.21 pancreas
-
Ovis aries
-
2.3.1.21 skeletal muscle
-
Ovis aries
-
2.3.1.21 skeletal muscle high level of mRNA Ovis aries
-
2.3.1.21 spleen
-
Ovis aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.21 palmitoyl-CoA + L-carnitine
-
Ovis aries CoA + L-palmitoylcarnitine
-
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Synonyms

EC Number Synonyms Comment Organism
2.3.1.21 CPT1A
-
Ovis aries
2.3.1.21 CPT1B
-
Ovis aries
2.3.1.21 L-CPT 1
-
Ovis aries
2.3.1.21 M-CPT 1
-
Ovis aries

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.21 0.01
-
IC50 for wild-type M-CPT-1 is 0.010 mM Ovis aries malonyl-CoA
2.3.1.21 0.0298
-
IC50 for wild-type L-CPT-1 is 0.0298 mM Ovis aries malonyl-CoA