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Literature summary extracted from

  • Mishiro, E.; Liu, M.Y.; Sakakibara, Y.; Suiko, M.; Liu, M.C.
    Zebrafish tyrosylprotein sulfotransferase: molecular cloning, expression, and functional characterization (2004), Biochem. Cell Biol., 82, 295-303.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.20 expression in COS-7 cells Danio rerio

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.20 Ca2+
-
 Danio rerio
2.8.2.20 Cd2+
-
 Danio rerio
2.8.2.20 Cu2+ 10 mM, complete inhibition Danio rerio
2.8.2.20 Fe2+ 10 mM, complete inhibition Danio rerio
2.8.2.20 Hg2+ 10 mM, complete inhibition Danio rerio
2.8.2.20 Pb2+
-
 Danio rerio
2.8.2.20 Zn2+ 10 mM, complete inhibition Danio rerio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.20 0.0052
-
 ATEYEFLDYDFL pH 5.8, 28°C Danio rerio
2.8.2.20 0.0085
-
 ATEFEYLDYDFL pH 5.8, 28°C Danio rerio
2.8.2.20 0.0315
-
 ATEFEFLDYDFL pH 5.8, 28°C Danio rerio
2.8.2.20 0.0359
-
 ATEYEYLDYDFL pH 5.8, 28°C Danio rerio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.20 membrane type II transmembrane protein Danio rerio 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.20 Co2+ 10 mM, stimulates Danio rerio
2.8.2.20 Mn2+ 20 mM, 2.5fold stimulation Danio rerio

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.20 Danio rerio Q6WZB4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.20 3'-phosphoadenylyl-sulfate + ATEFEFLDYDFL
-
 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylyl-sulfate + ATEFEYLDYDFL
-
 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylyl-sulfate + ATEYEFLDYDFL
-
 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.20 3'-phosphoadenylyl-sulfate + ATEYEYLDYDFL
-
 Danio rerio adenosine 3',5'-bisphosphate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.20 TPST
-
 Danio rerio
2.8.2.20 tyrosylprotein sulfotransferase
-
 Danio rerio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.20 5.8
-
-
 Danio rerio

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.20 5.5 6.5 pH 5.5: about 95% of maximal activity, pH 6.5: about 55% of maximal activity Danio rerio