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Literature summary extracted from

  • Nakamura, M.; Maki, S.; Amano, Y.; Ohkita, Y.; Niwa, K.; Hirano, T.; Ohmiya, Y.; Niwa, H.
    Firefly luciferase exhibits bimodal action depending on the luciferin chirality (2005), Biochem. Biophys. Res. Commun., 331, 471-475.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.7 D-luciferin inhibits CoA-ligase activity with L-luciferin, IC50: 0.135 mM against 0.1 mM L-luciferin Photinus pyralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.7 0.0982
-
 L-luciferin
-
 Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 dehydroluciferin + CoA
-
 Photinus pyralis dehydroluciferyl-CoA
-
 ?
1.13.12.7 L-luciferin + CoA + ATP L-luciferin is converted to luciferyl adenylate, and the adenyl group of luciferyl adenylate is then substituted to CoA-SH to give luciferyl-CoA. Even in presence of CoA-SH, D-luciferin is used for the light production reaction, but is not converted into luciferyl-CoA Photinus pyralis luciferyl-CoA + AMP + diphosphate
-
 ?
1.13.12.7 luciferin + O2 + ATP
-
 Photinus pyralis oxidized luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.12.7 0.135
-
 inhibits CoA-ligase activity with L-luciferin, IC50: 0.135 mM against 0.1 mM L-luciferin Photinus pyralis D-luciferin