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Literature summary extracted from

  • Etienne, F.; Resnick, L.; Sagher, D.; Brot, N.; Weissbach, H.
    Reduction of Sulindac to its active metabolite, sulindac sulfide: assay and role of the methionine sulfoxide reductase system (2003), Biochem. Biophys. Res. Commun., 312, 1005-1010.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.11 membrane
-
 Escherichia coli 16020
-
1.8.4.11 membrane
-
 Bos taurus 16020
-
1.8.4.11 microsome
-
 Escherichia coli
-
-
1.8.4.11 microsome
-
 Bos taurus
-
-
1.8.4.11 mitochondrion
-
 Escherichia coli 5739
-
1.8.4.11 mitochondrion
-
 Bos taurus 5739
-
1.8.4.12 membrane
-
 Bos taurus 16020
-
1.8.4.12 membrane membrane-associated enzyme MsrB Escherichia coli 16020
-
1.8.4.12 microsome
-
 Escherichia coli
-
-
1.8.4.12 microsome
-
 Bos taurus
-
-
1.8.4.12 mitochondrion
-
 Escherichia coli 5739
-
1.8.4.12 mitochondrion
-
 Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.11 sulindac + thioredoxin Bos taurus
-
 sulindac sulfide + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 sulindac + thioredoxin Escherichia coli activation of the antiinflammatory drug with anti-tumorigenic activity, which acts via inhibition of cyclooxygenases 1 and 2 sulindac sulfide + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 sulindac + thioredoxin Bos taurus
-
 sulindac sulfide + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 sulindac + thioredoxin Escherichia coli activation of the antiinflammatory drug with anti-tumorigenic activity, which acts via inhibition of cyclooxygenases 1 and 2 sulindac sulfide + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Bos taurus
-
 calf
-
1.8.4.11 Escherichia coli
-
 enzyme forms MsrA, and other enzyme forms, overview
-
1.8.4.12 Bos taurus
-
 calf
-
1.8.4.12 Escherichia coli
-
 enzyme forms MsrB, and other enzyme forms, e.g. Mem-R,S-Msr, overview
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.11 partially, cell fragmentation Bos taurus
1.8.4.11 recombinant MsrA Escherichia coli
1.8.4.12 partially, cell fragmentation Bos taurus
1.8.4.12 recombinant MsrB Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.11 brain
-
 Bos taurus
-
1.8.4.11 kidney highest sulindac reductase activity Bos taurus
-
1.8.4.11 liver
-
 Bos taurus
-
1.8.4.12 brain
-
 Bos taurus
-
1.8.4.12 kidney highest sulindac reductase activity Bos taurus
-
1.8.4.12 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.11 0.00019
-
 enzyme form MsrA Escherichia coli
1.8.4.12 0.000085
-
 enzyme form Mem-R,S-Msr Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 additional information substrate specificity of enzyme forms with S-form of free and protein-bound methionine sulfoxide, overview Escherichia coli ?
-
 ?
1.8.4.11 sulindac + thioredoxin
-
 Bos taurus sulindac sulfide + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 sulindac + thioredoxin activation of the antiinflammatory drug with anti-tumorigenic activity, which acts via inhibition of cyclooxygenases 1 and 2 Escherichia coli sulindac sulfide + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 sulindac + thioredoxin highest activity by enzyme MsrA, low activity by enzyme MsrA1 Escherichia coli sulindac sulfide + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 additional information substrate specificity of enzyme forms with R-form of free and protein-bound methionine sulfoxide, overview Escherichia coli ?
-
 ?
1.8.4.12 sulindac + thioredoxin
-
 Bos taurus sulindac sulfide + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 sulindac + thioredoxin activation of the antiinflammatory drug with anti-tumorigenic activity, which acts via inhibition of cyclooxygenases 1 and 2 Escherichia coli sulindac sulfide + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 sulindac + thioredoxin highest activity by a membrane bound enzyme form Mem-R,S-Msr, which preferentially reduces the R-substrate form, no activity by enzyme forms fRMsr, fSMsr, low activity by enzyme forms MsrB Escherichia coli sulindac sulfide + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 methionine sulfoxide reductase
-
 Bos taurus
1.8.4.11 sulindac reductase
-
 Bos taurus
1.8.4.12 methionine sulfoxide reductase
-
 Bos taurus
1.8.4.12 sulindac reductase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.11 37
-
 assay at Escherichia coli
1.8.4.11 37
-
 assay at Bos taurus
1.8.4.12 37
-
 assay at Escherichia coli
1.8.4.12 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.11 7.4
-
 assay at Escherichia coli
1.8.4.11 7.4
-
 assay at Bos taurus
1.8.4.12 7.4
-
 assay at Escherichia coli
1.8.4.12 7.4
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.11 thioredoxin
-
 Escherichia coli
1.8.4.11 thioredoxin
-
 Bos taurus
1.8.4.12 thioredoxin
-
 Escherichia coli
1.8.4.12 thioredoxin
-
 Bos taurus