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Literature summary extracted from

  • Mohamadin, A.M.; Abdel-Naim, A.B.
    In vitro activation of dibromoacetonitrile to cyanide: role of xanthine oxidase (2003), Arch. Toxicol., 77, 86-93.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.3.2 dithiothreitol enhance oxidation of dibromoacetonitrile by the hypoxanthine/xanthine oxidase/Fe system Bos taurus
1.17.3.2 glutathione enhances oxidation of dibromoacetonitrile by the hypoxanthine/xanthine oxidase/Fe system Bos taurus
1.17.3.2 N-acetyl-L-Cys enhance oxidation of dibromoacetonitrile by the hypoxanthine/xanthine oxidase/Fe system Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.3.2 allopurinol competitive inhibition of oxidation of dibromoacetonitrile by the hypoxanthine/xanthine oxidase/Fe system Bos taurus
1.17.3.2 desferrioxamine significant decrease in CN- formation from dibromoacetonitrile by the hypoxanthine/xanthine oxidase/Fe system Bos taurus
1.17.3.2 dimethylthiourea significant decrease in rate of oxidation of dibromoacetonitrile by the hypoxanthine/xanthine oxidase/Fe system Bos taurus
1.17.3.2 folic acid uncompetitive inhibition of oxidation of dibromoacetonitrile by the hypoxanthine/xanthine oxidase/Fe system Bos taurus
1.17.3.2 mannitol significant decrease in rate of oxidation of dibromoacetonitrile by the hypoxanthine/xanthine oxidase/Fe system Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.3.2 97
-
 dibromoacetonitrile
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.17.3.2 Bos taurus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.3.2 dibromoacetonitrile + H2O + O2
-
 Bos taurus ?
-
 r
1.17.3.2 additional information hydroxyl free radicals generated by the hypoxanthine/xanthine oxidase/Fe system are implicated in oxidation of dibromoacetonitrile to CN- Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.3.2 37
-
 conversion of dibromoacetonitrile to CN- Bos taurus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.17.3.2 30 45 30°C: about 60% of maximal activity, 45°C: about 75% of maximal activity, conversion of dibromoacetonitrile to CN- Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.3.2 8.5
-
 conversion of dibromoacetonitrile to CN- Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.3.2 6.5 9.5 pH 6.5: about 40% of maximal activity, pH 9.5: about 65% of maximal activity, conversion of dibromoacetonitrile to CN- Bos taurus