Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mampel, J.; Providenti, M.A.; Cook, A.M.
    Protocatechuate 4,5-dioxygenase from Comamonas testosteroni T-2: biochemical and molecular properties of a new subgroup within class III of extradiol dioxygenases (2005), Arch. Microbiol., 183, 130-139.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.8 0.055
-
 protocatechuate 30°C, pH 7.5 Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.8 18000
-
 alpha,beta, 4 * 18000 + 4 * 31000, SDS-PAGE Comamonas testosteroni
1.13.11.8 31000
-
 alpha,beta, 4 * 18000 + 4 * 31000, SDS-PAGE Comamonas testosteroni
1.13.11.8 180000
-
 gel filtration Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.8 protocatechuate + O2 Comamonas testosteroni
-
 4-carboxy-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.8 protocatechuate + O2 Comamonas testosteroni T-2
-
 4-carboxy-2-hydroxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.8 Comamonas testosteroni Q8RNX9 beta-subunit; T-2
-
1.13.11.8 Comamonas testosteroni Q8RNY0 alpha-subunit; T-2
-
1.13.11.8 Comamonas testosteroni T-2 Q8RNX9 beta-subunit; T-2
-
1.13.11.8 Comamonas testosteroni T-2 Q8RNY0 alpha-subunit; T-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.8 heat treatment, DEAE-Sepharose, superdex 200, Resource Q Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.8 0.1
-
-
 Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.8 gallate + O2
-
 Comamonas testosteroni ?
-
 ?
1.13.11.8 gallate + O2
-
 Comamonas testosteroni T-2 ?
-
 ?
1.13.11.8 protocatechuate + O2
-
 Comamonas testosteroni 4-carboxy-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.8 protocatechuate + O2
-
 Comamonas testosteroni T-2 4-carboxy-2-hydroxymuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.8 octamer alpha,beta, 4 * 18000 + 4 * 31000, SDS-PAGE Comamonas testosteroni

Synonyms

EC Number Synonyms Comment Organism
1.13.11.8 PmdAB
-
 Comamonas testosteroni