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Literature summary extracted from

  • Yow, G.Y.; Uo, T.; Yoshimura, T.; Esaki, N.
    D-amino acid N-acetyltransferase of Saccharomyces cerevisiae: a close homologue of histone acetyltransferase Hpa2p acting exclusively on free D-amino acids (2004), Arch. Microbiol., 182, 396-403.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.36 gene HPA3, functional overexpression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.36 CoA competitive against acetyl-CoA, noncompetitive against D-tryptophan Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.36 additional information
-
 additional information kinetics Saccharomyces cerevisiae
2.3.1.36 0.034
-
 D-serine 30°C, recombinant enzyme Saccharomyces cerevisiae
2.3.1.36 0.054
-
 D-alanine 30°C, recombinant enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.36 18000
-
 x * 21000, recombinant intact Hpa3p, SDS-PAGE, x * 18000, recombinant N-terminally truncated enzyme, SDS-PAGE Saccharomyces cerevisiae
2.3.1.36 21000
-
 x * 21000, recombinant intact Hpa3p, SDS-PAGE, x * 18000, recombinant N-terminally truncated enzyme, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.36 acetyl-CoA + a D-amino acid Saccharomyces cerevisiae
-
 CoA + an N-acetyl-D-amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.36 Saccharomyces cerevisiae
-
 gene HPA3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.36 native enzyme from dry yeast, to homogeneity in several steps, recombinant enzyme from Escherichia coli in a procedure similar to the purification of the native enzyme to 95% purity Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.36 acetyl-CoA + a D-amino acid = CoA + an N-acetyl-D-amino acid ordered bi bi mechanism with acetyl-CoA being the first substrate to be bound and CoA the last product to be released, involving acetylation of Cys304 Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.36 additional information
-
 substrate specificity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.36 acetyl-CoA + a D-amino acid
-
 Saccharomyces cerevisiae CoA + an N-acetyl-D-amino acid
-
 ?
2.3.1.36 acetyl-CoA + a D-amino acid substrate specificity, overview Saccharomyces cerevisiae CoA + an N-acetyl-D-amino acid
-
 ?
2.3.1.36 acetyl-CoA + D-alanine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-alanine
-
 ?
2.3.1.36 acetyl-CoA + D-alpha-aminobutyrate
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-alpha-aminobutyrate
-
 ?
2.3.1.36 acetyl-CoA + D-arginine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-arginine
-
 ?
2.3.1.36 acetyl-CoA + D-asparagine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-asparagine
-
 ?
2.3.1.36 acetyl-CoA + D-glutamine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-glutamine
-
 ?
2.3.1.36 acetyl-CoA + D-lysine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-lysine
-
 ?
2.3.1.36 acetyl-CoA + D-methionine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-methionine
-
 ?
2.3.1.36 acetyl-CoA + D-norleucine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-norleucine
-
 ?
2.3.1.36 acetyl-CoA + D-norvaline
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-norvaline
-
 ?
2.3.1.36 acetyl-CoA + D-phenylalanine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-phenylalanine
-
 ?
2.3.1.36 acetyl-CoA + D-serine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-serine
-
 ?
2.3.1.36 acetyl-CoA + D-threonine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-threonine
-
 ?
2.3.1.36 acetyl-CoA + D-tryptophan
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-tryptophan
-
 ?
2.3.1.36 acetyl-CoA + D-valine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-valine
-
 ?
2.3.1.36 additional information no activity with L-lysine, D-proline, and D-aspartate, D-glutamate is a poor substrate Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.36 ? x * 21000, recombinant intact Hpa3p, SDS-PAGE, x * 18000, recombinant N-terminally truncated enzyme, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.3.1.36 DNT
-
 Saccharomyces cerevisiae
2.3.1.36 Hpa3p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.36 30
-
 assay at Saccharomyces cerevisiae