Literature summary extracted from
Thiemann, V.; Donges, C.; Prowe, S.G.; Sterner, R.; Antranikian, G.
Characterisation of a thermoalkali-stable cyclodextrin glycosyltransferase from the anaerobic thermoalkaliphilic bacterium Anaerobranca gottschalkii (2004), Arch. Microbiol., 182, 226-235.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.4.1.19 |
expression in Escherichia coli |
Anaerobranca gottschalkii |
General Stability
EC Number |
General Stability |
Organism |
---|
2.4.1.19 |
Ca2+ stabilizes against thermal inactivation, 5 mM CaCl2 shifts the apparent melting point from 60°C to 69°C |
Anaerobranca gottschalkii |
2.4.1.19 |
high substrate concentrations stabilize against thermal inactivation |
Anaerobranca gottschalkii |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.4.1.19 |
78000 |
- |
x * 78000, SDS-PAGE |
Anaerobranca gottschalkii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.4.1.19 |
Anaerobranca gottschalkii |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.4.1.19 |
recombinant |
Anaerobranca gottschalkii |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.4.1.19 |
308 |
- |
- |
Anaerobranca gottschalkii |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.4.1.19 |
amylopectin + glycosyl acceptor |
alpha-cyclodextrin is preferentially produced. With a longer incubation period, the alpha-cyclodextrin to beta-cyclodextrin ratio declines |
Anaerobranca gottschalkii |
alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin |
larger cyclodextrins (>8 glucose units) are formed in the initial reaction period |
? |
|
2.4.1.19 |
amylose + glycosyl acceptor |
alpha-cyclodextrin is preferentially produced. With a longer incubation period, the alpha-cyclodextrin to beta-cyclodextrin ratio declines |
Anaerobranca gottschalkii |
alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin |
larger cyclodextrins (>8 glucose units) are formed in the initial reaction period |
? |
|
2.4.1.19 |
additional information |
increasing substrate concentrations (0.5%-20.0%) and glucans containing branching points (alpha-1,6-glycosidic linkages) shift the product pattern to: beta-cyclodextrin > alpha-cyclodextrin > gamma-cyclodextrin |
Anaerobranca gottschalkii |
? |
- |
? |
|
2.4.1.19 |
native starch + glycosyl acceptor |
alpha-cyclodextrin is preferentially produced. With a longer incubation period, the alpha-cyclodextrin to beta-cyclodextrin ratio declines |
Anaerobranca gottschalkii |
alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin |
larger cyclodextrins (>8 glucose units) are formed in the initial reaction period |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.4.1.19 |
? |
x * 78000, SDS-PAGE |
Anaerobranca gottschalkii |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.4.1.19 |
CGTase |
- |
Anaerobranca gottschalkii |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.4.1.19 |
65 |
- |
- |
Anaerobranca gottschalkii |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
2.4.1.19 |
55 |
70 |
55°C: about 75% of maximal activity, 70°C: about 90% of maximal activity |
Anaerobranca gottschalkii |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
2.4.1.19 |
60 |
- |
5 mM CaCl2 shifts the apparent melting point from 60°C to 69°C |
Anaerobranca gottschalkii |
2.4.1.19 |
69 |
- |
5 mM CaCl2 shifts the apparent melting point from 60°C to 69°C |
Anaerobranca gottschalkii |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.4.1.19 |
6 |
9 |
- |
Anaerobranca gottschalkii |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
2.4.1.19 |
5 |
10 |
pH 5.0: about 70% of maximal activity, pH 10.0: about 50% of maximal activity |
Anaerobranca gottschalkii |