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Literature summary extracted from

  • Curley, P.; van der Does, C.; Driessen, A.J.; Kok, J.; van Sinderen, D.
    Purification and characterisation of a lactococcal aminoacylase (2003), Arch. Microbiol., 179, 402-408.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.14 expressed in Escherichia coli Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.14 Cd2+ 50% inhibition at 1 mM Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 1.2
-
 N-alpha-acetyl-L-alanine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 1.4
-
 N-alpha-chloroacetyl-L-phenylalanine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 1.7
-
 N-alpha-acetyl-L-phenylalanine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 1.9
-
 N-alpha-acetyl-L-tyrosine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 2.3
-
 N-alpha-acetyl-L-leucine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 2.4
-
 N-alpha-acetyl-L-tryptophan pH 7.0, 30°C Lactococcus lactis
3.5.1.14 2.5
-
 N-alpha-acetyl-L-valine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 2.7
-
 N-alpha-acetyl-L-phenylalanine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 3.1
-
 N-alpha-acetyl-L-isoleucine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 4
-
 N-alpha-acetyl-L-threonine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 4.2
-
 N-alpha-acetyl-L-serine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 5
-
 N-alpha-acetyl-L-proline pH 7.0, 30°C Lactococcus lactis
3.5.1.14 5
-
 N-alpha-acetyl-L-aspartate pH 7.0, 30°C Lactococcus lactis
3.5.1.14 5.9
-
 N-acetyl-L-glutamic acid pH 7.0, 30°C Lactococcus lactis
3.5.1.14 6.7
-
 N-alpha-acetyl-L-lysine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 7.7
-
 N-alpha-acetyl-L-methionine pH 7.0, 30°C Lactococcus lactis
3.5.1.14 9.1
-
 N-alpha-acetyl-L-cysteine pH 7.0, 30°C Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.14 Co2+ required, activity can be increased significantly by the addition of Co2+ Lactococcus lactis
3.5.1.14 Mn2+ activity can be increased by the addition of Mn2+ Lactococcus lactis
3.5.1.14 Zn2+ required Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.14 42000
-
 2 * 42000, SDS-PAGE Lactococcus lactis
3.5.1.14 44147
-
 2 * 44147, deduced from nucleotide sequence Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Lactococcus lactis
-
-
-
3.5.1.14 Lactococcus lactis MG1363
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 additional information N-acyl-D-alanine is not substrate Lactococcus lactis ?
-
 ?
3.5.1.14 additional information N-acyl-D-alanine is not substrate Lactococcus lactis MG1363 ?
-
 ?
3.5.1.14 N-alpha-acetyl-L-alanine + H2O
-
 Lactococcus lactis acetate + L-alanine
-
 ?
3.5.1.14 N-alpha-acetyl-L-alanine + H2O
-
 Lactococcus lactis MG1363 acetate + L-alanine
-
 ?
3.5.1.14 N-alpha-acetyl-L-aspartate + H2O
-
 Lactococcus lactis acetate + L-aspartate
-
 ?
3.5.1.14 N-alpha-acetyl-L-aspartate + H2O
-
 Lactococcus lactis MG1363 acetate + L-aspartate
-
 ?
3.5.1.14 N-alpha-acetyl-L-cysteine + H2O
-
 Lactococcus lactis acetate + L-cysteine
-
 ?
3.5.1.14 N-alpha-acetyl-L-cysteine + H2O
-
 Lactococcus lactis MG1363 acetate + L-cysteine
-
 ?
3.5.1.14 N-alpha-acetyl-L-glutamate + H2O
-
 Lactococcus lactis acetate + L-glutamate
-
 ?
3.5.1.14 N-alpha-acetyl-L-glutamate + H2O
-
 Lactococcus lactis MG1363 acetate + L-glutamate
-
 ?
3.5.1.14 N-alpha-acetyl-L-isoleucine + H2O
-
 Lactococcus lactis acetate + L-isoleucine
-
 ?
3.5.1.14 N-alpha-acetyl-L-leucine + H2O
-
 Lactococcus lactis acetate + L-leucine
-
 ?
3.5.1.14 N-alpha-acetyl-L-lysine + H2O
-
 Lactococcus lactis acetate + L-lysine
-
 ?
3.5.1.14 N-alpha-acetyl-L-methionine + H2O
-
 Lactococcus lactis acetate + L-methionine
-
 ?
3.5.1.14 N-alpha-acetyl-L-phenylalanine + H2O
-
 Lactococcus lactis acetate + L-phenylalanine
-
 ?
3.5.1.14 N-alpha-acetyl-L-proline + H2O
-
 Lactococcus lactis acetate + L-proline
-
 ?
3.5.1.14 N-alpha-acetyl-L-serine + H2O
-
 Lactococcus lactis acetate + L-serine
-
 ?
3.5.1.14 N-alpha-acetyl-L-threonine + H2O
-
 Lactococcus lactis acetate + L-threonine
-
 ?
3.5.1.14 N-alpha-acetyl-L-tryptophan + H2O
-
 Lactococcus lactis acetate + L-tryptophan
-
 ?
3.5.1.14 N-alpha-acetyl-L-tyrosine + H2O
-
 Lactococcus lactis acetate + L-tyrosine
-
 ?
3.5.1.14 N-alpha-acetyl-L-valine + H2O
-
 Lactococcus lactis acetate + L-valine
-
 ?
3.5.1.14 N-alpha-benzoyl-L-phenylalanine + H2O
-
 Lactococcus lactis benzoate + L-phenylalanine
-
 ?
3.5.1.14 N-alpha-chloroacetyl-L-phenylalanine + H2O
-
 Lactococcus lactis chloroacetate + L-phenylalanine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.14 dimer 2 * 42000, SDS-PAGE Lactococcus lactis
3.5.1.14 dimer 2 * 44147, deduced from nucleotide sequence Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.14 30
-
-
 Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.14 7
-
-
 Lactococcus lactis