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Literature summary extracted from

  • Zhou, W.; Nes, W.D.
    Sterol methyltransferase2: purification, properties, and inhibition (2003), Arch. Biochem. Biophys., 420, 18-34.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.142 expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.142 24(28)-methylenecycloartanol competitive with cycloartenol Arabidopsis thaliana
2.1.1.142 24beta-methylcycloartanol competitive with cycloartenol Arabidopsis thaliana
2.1.1.142 25-Azacycloartenol competitive with cycloartenol or 24(28)-methylenelophenol; noncompetitive Arabidopsis thaliana
2.1.1.142 26,27-dehydrozymosterol mechanism-based inhibitor, specific covalent modification of SMT2 Arabidopsis thaliana
2.1.1.142 citrostadienol competitive with cycloartenol or 24(28)-methylenelophenol Arabidopsis thaliana
2.1.1.142 sitosterol competitive with cycloartenol or 24(28)-methylenelophenol Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.142 0.015
-
 26,27-dehydrozymosterol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.016
-
 fecosterol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.023
-
 dehydropollinastanol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.028
-
 Zymosterol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.03
-
 31-norcycloartenol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.03
-
 24(28)-methylenelophenol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.03
-
 4alpha-methylfecosterol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.03
-
 4alpha-methylzymosterol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.03
-
 cholest-7,24-dienol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.035
-
 Cycloartenol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.035
-
 24(28)-methylenelanosterol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.035
-
 24(28)-methylenecholest-7-enol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.036
-
 24(28)-methylenecholesterol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.038
-
 desmosterol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.038
-
 obtusifoliol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.04
-
 24(28)-methylenecycloartanol pH 7.5, 35°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.142 40000
-
 4 * 40000, SDS-PAGE Arabidopsis thaliana
2.1.1.142 160000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.142 cycloartenol + S-adenosyl-L-methionine Arabidopsis thaliana SMT2 has a position-specific substrate specificity for DELTA24(25)-sterols and contains a single active center to catalyze the consecutive C1-transfer activities by substrate reaction channels similar to the fungal SMT1 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.142 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.142
-
 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.142 additional information
-
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.142 24(28)-methylenecholest-7-enol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 24(28)-methylenecholesterol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 24(28)-methylenecycloartanol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 24(28)-methylenelanosterol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 24(28)-methylenelophenol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana additional information a mixture of three stereochemical related products with DELTA24(28)Z-ethylidene, DELTA24(28)-ethylidene and DELTA25(27)-24beta-ethyl side chains ?
2.1.1.142 26,27-dehydrozymosterol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana 26-homocholesta-8(9),23(24)E,26(26')-trienol + 26-homocholesta-8(9),26(26')-3beta,24beta-dienol
-
 ?
2.1.1.142 31-norcycloartenol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 4alpha-methylfecosterol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 4alpha-methylzymosterol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 cholest-7,24-dienol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 cycloartenol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 cycloartenol + S-adenosyl-L-methionine SMT2 has a position-specific substrate specificity for DELTA24(25)-sterols and contains a single active center to catalyze the consecutive C1-transfer activities by substrate reaction channels similar to the fungal SMT1 Arabidopsis thaliana 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 dehydropollinastanol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 desmosterol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 fecosterol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 additional information no activity with cycloartanol, 3-deoxycycloartenol, or cyclolaudenol Arabidopsis thaliana ?
-
 ?
2.1.1.142 obtusifoliol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ?
-
 ?
2.1.1.142 zymosterol + S-adenosyl-L-methionine
-
 Arabidopsis thaliana fecosterol + S-adenosyl-L-homocysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.142 tetramer 4 * 40000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.1.1.142 SMT2
-
 Arabidopsis thaliana
2.1.1.142 sterol methyltransferase2
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.142 0.001
-
 26,27-dehydrozymosterol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.01
-
 Cycloartenol pH 7.5, 35°C Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.142 0.00002
-
 25-Azacycloartenol pH 7.5, 35°C, substrate: cycloartenol Arabidopsis thaliana
2.1.1.142 0.045
-
 citrostadienol pH 7.4, 35°C, substrate: 24(28)-methylenelophenol Arabidopsis thaliana
2.1.1.142 0.049
-
 26,27-dehydrozymosterol pH 7.5, 35°C Arabidopsis thaliana
2.1.1.142 0.05
-
 citrostadienol pH 7.4, 35°C, substrate cycloartenol Arabidopsis thaliana
2.1.1.142 0.15
-
 24beta-methylcycloartanol pH 7.4, 35°C, substrate cycloartenol Arabidopsis thaliana
2.1.1.142 0.2
-
 sitosterol pH 7.5, 35°C, substrate: cycloartenol Arabidopsis thaliana
2.1.1.142 0.207
-
 sitosterol pH 7.4, 35°C, substrate: 24(28)-methylenelophenol Arabidopsis thaliana
2.1.1.142 0.25
-
 24(28)-methylene cycloartanol pH 7.4, 35°C, substrate cycloartenol Arabidopsis thaliana