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Literature summary extracted from

  • Miller, L.A.; Baumgart, L.E.; Chew, G.H.; deLong, M.A.; Galloway, L.C.; Jung, K.W.; Merkler, K.A.; Nagle, A.S.; Poore, D.D.; Yoon, C.H.; Merkler, D.J.
    Glutathione, S-substituted glutathiones, and leukotriene C4 as substrates for peptidylglycine alpha-amidating monooxygenase (2003), Arch. Biochem. Biophys., 412, 3-12.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.3 0.016
-
 S-decyl-glutathione pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.027
-
 S-(4-nitrobenzyl)-glutathione pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.032
-
 S-octyl-glutathione pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.035
-
 leukotriene C4 pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.035
-
 S-hexyl-glutathione pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.067
-
 S-butyl-glutathione pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.1
-
 N-decanoylglycine pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.11
-
 S-propyl-glutathione pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.13
-
 glutathione pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.22
-
 D-Tyr-Val-Gly pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.23
-
 S-ethyl-glutathione pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.27
-
 S-methyl-glutathione pH 6.0, 37°C Rattus norvegicus
1.14.17.3 0.42
-
 N-hexanoylglycine pH 6.0, 37°C Rattus norvegicus
1.14.17.3 1.1
-
 S-(1,2-dicarboxyethyl)-glutathione pH 6.0, 37°C Rattus norvegicus
1.14.17.3 2.1
-
 gamma-Glu-Gly-Gly pH 6.0, 37°C Rattus norvegicus
1.14.17.3 9.3
-
 N-acetylglycine pH 6.0, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.3 Cu2+ bifunctional copper- and zinc-dependent enzyme Rattus norvegicus
1.14.17.3 Zn2+ bifunctional copper- and zinc-dependent enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.3 glutathione + ascorbate + O2 Rattus norvegicus
-
 gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 additional information Rattus norvegicus cleavage of CN bond in N-acetylated glycines, R-CO-NH-CH2-COOH, e.g. found in neuropeptide prohormones, inhibition of the enzyme leads to a decrease in alpha-aminated peptide production and accumulation of glycine-extended precursors ?
-
 ?
1.14.17.3 R-CO-NH-CH2-COOH + ascorbate + O2 Rattus norvegicus
-
 R-CO-NH2 + CHO-COOH + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.3 Rattus norvegicus
-
 bifunctional copper- and zinc-dependent enzyme
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.17.3 [peptide]-glycine + 2 ascorbate + O2 = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H2O cleavage of CN bond in N-acetylated glycines, R-CO-NH-CH2-COOH, e.g. found in neuropeptide prohormones Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.3 medullary thyroid carcinoma cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.3 additional information
-
-
 Rattus norvegicus
1.14.17.3 3
-
 purified recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.3 D-Tyr-Val-Gly + ascorbate + O2
-
 Rattus norvegicus D-Tyr-Val-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 gamma-Glu-Gly-Gly + ascorbate + O2
-
 Rattus norvegicus gamma-Glu-Gly-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 glutathione + ascorbate + O2
-
 Rattus norvegicus gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 leukotriene C4 + ascorbate + O2
-
 Rattus norvegicus ? + dehydroascorbate + H2O
-
 ?
1.14.17.3 additional information cleavage of CN bond in N-acetylated glycines, R-CO-NH-CH2-COOH, e.g. found in neuropeptide prohormones, inhibition of the enzyme leads to a decrease in alpha-aminated peptide production and accumulation of glycine-extended precursors Rattus norvegicus ?
-
 ?
1.14.17.3 additional information substrate specificity, cleavage of CN bond in N-acetylated glycines, R-CO-NH-CH2-COOH, e.g. found in neuropeptide prohormones Rattus norvegicus ?
-
 ?
1.14.17.3 N-acetylglycine + ascorbate + O2
-
 Rattus norvegicus acetylamine + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 N-decanoylglycine + ascorbate + O2
-
 Rattus norvegicus decanoylamine + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 N-hexanoylglycine + ascorbate + O2
-
 Rattus norvegicus hexanoylamine + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 R-CO-NH-CH2-COOH + ascorbate + O2
-
 Rattus norvegicus R-CO-NH2 + CHO-COOH + dehydroascorbate + H2O
-
 ?
1.14.17.3 R-CO-NH-CH2-COOH + ascorbate + O2 via hydroxylated reaction intermediates Rattus norvegicus R-CO-NH2 + CHO-COOH + dehydroascorbate + H2O
-
 ?
1.14.17.3 S-(1,2-dicarboxyethyl)-glutathione + ascorbate + O2
-
 Rattus norvegicus S-(1,2-dicarboxyethyl)-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 S-(4-nitrobenzyl)-glutathione + ascorbate + O2
-
 Rattus norvegicus S-(4-nitrobenzyl)-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 S-butyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-butyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 S-decyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-decyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 S-ethyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-ethyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 S-hexyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-hexyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 S-methyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-methyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 S-octyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-octyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?
1.14.17.3 S-propyl-glutathione + ascorbate + O2
-
 Rattus norvegicus S-propyl-gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.17.3 PAM
-
 Rattus norvegicus
1.14.17.3 peptidylglycine alpha-amidating monooxygenase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.3 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.3 6
-
 assay at Rattus norvegicus