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Literature summary extracted from
- Peptide methionine sulfoxide reductase: structure, mechanism of action, and biological function (2002), Arch. Biochem. Biophys., 397, 172-178.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | gene msrA | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | C52S | site-directed mutagenesis, reduced activity compared to the wild-type, no complementation of a msrA knockout mutant | Escherichia coli |
| 1.8.4.11 | additional information | a knockout mutant shows reduced ability to attach to host lung and vein epithelial cells | Streptococcus pneumoniae |
| 1.8.4.11 | additional information | construction of knockout mutants which show higher sensitivity to hydrogen peroxide compared to wild-type cells which can be compensated by complementation with the wild-type msrA gene from either Escherichia coli or Mycobacterium tuberculosis, but a mutant C52S msrA gene cannot restore activity in the knockout mutant strain, mutants show reduced type 1 fimbriae-mediated mannose-dependent agglutination of erythrocytes | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | calmodulin-L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | - |
calmodulin-L-methionine + thioredoxin disulfide + H2O | - |
? |  |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | Streptococcus pneumoniae | - |
L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | substrates are several peptides and proteins, overview | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | additional information | Escherichia coli | the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function | ? | - |
? | |
| 1.8.4.11 | additional information | Streptococcus pneumoniae | the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function | ? | - |
? | |
| 1.8.4.11 | ribosomal protein L12-L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | - |
ribosomal protein L12-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | calmodulin-L-methionine (R)-sulfoxide + thioredoxin | Escherichia coli | - |
calmodulin-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | Escherichia coli | substrates are peptides and proteins | L-methionine + thioredoxin disulfide + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | Escherichia coli | - |
- |
- |
| 1.8.4.11 | Streptococcus pneumoniae | - |
- |
- |
| 1.8.4.12 | Escherichia coli | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | reaction mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.12 | additional information | - |
in vivo MsrB activity is below detection limit | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | calmodulin-L-methionine (S)-sulfoxide + thioredoxin | - |
Escherichia coli | calmodulin-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + 2 dithiothreitol | - |
Escherichia coli | L-methionine + dithiothreitol disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | - |
Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | - |
Streptococcus pneumoniae | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | substrates are several peptides and proteins, overview | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | additional information | MsrA is specific for the S-form of the substrate | Escherichia coli | ? | - |
? | |
| 1.8.4.11 | additional information | the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function | Escherichia coli | ? | - |
? | |
| 1.8.4.11 | additional information | the enzyme is important in protection of the cell against oxidative damage by oxidation of methionine residues in proteins, biological function | Streptococcus pneumoniae | ? | - |
? | |
| 1.8.4.11 | ribosomal protein L12-L-methionine (S)-sulfoxide + thioredoxin | - |
Escherichia coli | ribosomal protein L12-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | calmodulin-L-methionine (R)-sulfoxide + thioredoxin | - |
Escherichia coli | calmodulin-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + dithiothreitol | - |
Escherichia coli | L-methionine + dithiothreitol disulfide | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | - |
Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | substrates are peptides and proteins | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | additional information | MsrB is specific for the R-form of the substrate | Escherichia coli | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | MsrA | - |
Escherichia coli |
| 1.8.4.11 | MsrA | - |
Streptococcus pneumoniae |
| 1.8.4.11 | peptide methionine sulfoxide reductase | - |
Escherichia coli |
| 1.8.4.11 | peptide methionine sulfoxide reductase | - |
Streptococcus pneumoniae |
| 1.8.4.12 | MsrB | - |
Escherichia coli |
| 1.8.4.12 | peptide methionine sulfoxide reductase | - |
Escherichia coli |
| 1.8.4.12 | YeaA | - |
Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | dithiothreitol | utilized in vitro | Escherichia coli | |
| 1.8.4.11 | thioredoxin | physiologic cofactor | Escherichia coli | |
| 1.8.4.12 | dithiothreitol | utilized in vitro | Escherichia coli | |
| 1.8.4.12 | thioredoxin | physiologic cofactor | Escherichia coli | |
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