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Literature summary extracted from

  • Sperling, P.; Libisch, B.; Zahringer, U.; Napier, J.A.; Heinz, E.
    Functional identification of a DELTA8-sphingolipid desaturase from Borago officinalis (2001), Arch. Biochem. Biophys., 388, 293-298.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.4 expression of this open-reading frame in Saccharomyces cerevisiae results in the formation of DELTA-trans/cis-phytosphingenines not present in wild-type cells Borago officinalis
1.14.19.29 expressed in Saccharomyces cerevisiae strain w303-1a Borago officinalis
1.14.19.29 expression of this openreading frame in Saccharomyces cerevisiae results in the formation of DELTA8-trans/cis-phytosphingenines not present in wild-type cells Borago officinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.29 C18-phytosphinganine + ferrocytochrome b5 + O2 + H+ Borago officinalis
-
 C18-8-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 sphinganine + ferrocytochrome b5 + O2 + H+ Borago officinalis
-
 8-sphingenine + ferricytochrome b5 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.4 Borago officinalis Q9FR82
-
-
1.14.19.29 Borago officinalis
-
-
-
1.14.19.29 Borago officinalis Q9FR82
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.19.29 a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O (1) Borago officinalis
a
1.14.19.29 a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O (2) Borago officinalis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.4 leaf
-
 Borago officinalis
-
1.14.19.29 leaf primarily expressed in young leaves Borago officinalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.29 C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
-
 Borago officinalis C18-8-phytosphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 sphinganine + ferrocytochrome b5 + O2 + H+
-
 Borago officinalis 8-sphingenine + ferricytochrome b5 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.4 DELTA8-sphingolipid desaturase
-
 Borago officinalis
1.14.19.29 8 fatty acid desaturase
-
 Borago officinalis
1.14.19.29 8-sphingolipid desaturase
-
 Borago officinalis
1.14.19.29 DELTA8-sphingolipid desaturase
-
 Borago officinalis
1.14.19.29 DES8
-
 Borago officinalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.29 cytochrome b5
-
 Borago officinalis