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Literature summary extracted from

  • Fraaije, M.W.; Wu, J.; Heuts, D.P.; van Hellemond, E.W.; Spelberg, J.H.; Janssen, D.B.
    Discovery of a thermostable Baeyer-Villiger monooxygenase by genome mining (2005), Appl. Microbiol. Biotechnol., 66, 393-400.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.92 expression in Escherichia coli Thermobifida fusca

General Stability

EC Number General Stability Organism
1.14.13.92 PAMO that has been activated at 50°C can be stored for several days at room temperature without any loss in activity Thermobifida fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.92 0.059
-
 phenylacetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 0.26
-
 2-dodecanone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 0.36
-
 benzylacetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 0.83
-
 (2-methylphenyl)acetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 0.86
-
 methyl 4-tolylsulfide 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 2.2
-
 4-hydroxyacetophenone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 15
-
 bicyclohept-2-en-6-one 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.92 65000
-
 1 * 65000, SDS-PAGE, small amount of approx. 7% is present as a dimer Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.92 Thermobifida fusca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.92 recombinant PAMO Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.92 (2-methylphenyl)acetone + NADPH + O2
-
 Thermobifida fusca ? + NADP+ + O2
-
 ?
1.14.13.92 2-dodecanone + NADPH + O2
-
 Thermobifida fusca ? + NADP+
-
 ?
1.14.13.92 4-hydroxyacetophenone + NADPH + O2
-
 Thermobifida fusca acetic acid 4-hydroxyphenyl ester + NADP+ + O2
-
 ?
1.14.13.92 benzylacetone + NADPH + O2
-
 Thermobifida fusca ? + NADP+ + H2O
-
 ?
1.14.13.92 bicyclohept-2-en-6-one + NADPH + O2
-
 Thermobifida fusca ? + NADP+ + O2
-
 ?
1.14.13.92 ethionamide + NADPH + O2
-
 Thermobifida fusca ? + NADP+ + O2
-
 ?
1.14.13.92 methyl 4-tolylsulfide + NADPH + O2
-
 Thermobifida fusca ? + NADP+ + O2
-
 ?
1.14.13.92 phenylacetone + NADPH + H+ + O2
-
 Thermobifida fusca benzyl acetate + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.92 monomer 1 * 65000, SDS-PAGE, small amount of approx. 7% is present as a dimer Thermobifida fusca

Synonyms

EC Number Synonyms Comment Organism
1.14.13.92 Baeyer-Villiger monooxygenase
-
 Thermobifida fusca
1.14.13.92 BVMO
-
 Thermobifida fusca
1.14.13.92 PAMO
-
 Thermobifida fusca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.92 70
-
 4fold higher activity when compared with the activity at 25°C Thermobifida fusca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.92 52
-
 50% loss of activity after 24 h and 48 h in the absence and presence of FAD, respectively Thermobifida fusca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.92 0.23
-
 2-dodecanone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 0.34
-
 4-hydroxyacetophenone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 1.1
-
 bicyclohept-2-en-6-one 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 1.8
-
 benzylacetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 1.9
-
 phenylacetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 2
-
 (2-methylphenyl)acetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.14.13.92 2.1
-
 methyl 4-tolylsulfide 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.92 8
-
-
 Thermobifida fusca

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.92 7 9 more than 80% of maximal activity Thermobifida fusca

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.92 FAD
-
 Thermobifida fusca
1.14.13.92 NADPH can not be replaced by NADH Thermobifida fusca