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Literature summary extracted from

  • Smith, C.A.; Hyman, M.R.
    Oxidation of methyl tert-butyl ether by alkane hydroxylase in dicyclopropylketone-induced and n-octane-grown Pseudomonas putida GPo1 (2004), Appl. Environ. Microbiol., 70, 4544-4550.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.3 1,7-octadiyne inhibits oxidation of methyl tert-butyl ether; putative mechanism-based inactivator, strongly inhibitory at 0.1% (v/v) Pseudomonas putida
1.14.15.3 n-alkanes C5-C10 inhibits oxidation of methyl tert-butyl ether, weaker inhibition at longer chain length Pseudomonas putida
1.14.15.3 n-butane inhibits oxidation of methyl tert-butyl ether Pseudomonas putida
1.14.15.3 n-decane 0.1% (v/v) Pseudomonas putida
1.14.15.3 n-dodecane 0.1% (v/v) Pseudomonas putida
1.14.15.3 n-heptane 0.1% (v/v) Pseudomonas putida
1.14.15.3 n-hexane 0.1% (v/v) Pseudomonas putida
1.14.15.3 n-nonane 0.1% (v/v) Pseudomonas putida
1.14.15.3 n-octane 0.1% (v/v) Pseudomonas putida
1.14.15.3 n-pentane 0.1% (v/v) Pseudomonas putida
1.14.15.3 propane inhibits oxidation of methyl tert-butyl ether Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Pseudomonas putida
-
n-alkanol + NAD(P)+ + H2O
-
?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Pseudomonas putida GPo1 / ATCC29347
-
n-alkanol + NAD(P)+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Pseudomonas putida
-
-
-
1.14.15.3 Pseudomonas putida
-
grown in medium containing dicyclopropylketone
-
1.14.15.3 Pseudomonas putida GPo1 / ATCC29347
-
-
-
1.14.15.3 Pseudomonas putida GPo1 / ATCC29347
-
grown in medium containing dicyclopropylketone
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.3 culture condition:dicyclopropylketone-grown cell
-
Pseudomonas putida
-
1.14.15.3 culture condition:n-octane-grown cell
-
Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.3 0.00012
-
using ethyl tert-butyl ether as a substrate Pseudomonas putida
1.14.15.3 0.00313
-
using tert-amyl methyl ether as a substrate Pseudomonas putida
1.14.15.3 0.0077
-
pH 7.0, 30°C Pseudomonas putida
1.14.15.3 0.00773
-
using methyl tert-butyl ether as a substrate Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 ethyl tert-butyl ether + NAD(P)H + H+ + O2
-
Pseudomonas putida tert-butyl-alcohol + NAD(P)+ + H2O + acetaldehyde
-
?
1.14.15.3 ethyl tert-butyl ether + NAD(P)H + H+ + O2
-
Pseudomonas putida GPo1 / ATCC29347 tert-butyl-alcohol + NAD(P)+ + H2O + acetaldehyde
-
?
1.14.15.3 methyl tert-butyl ether + NAD(P)H + H+ + O2
-
Pseudomonas putida tert-butyl-alcohol + NAD(P)+ + H2O
-
?
1.14.15.3 methyl tert-butyl ether + NAD(P)H + H+ + O2
-
Pseudomonas putida GPo1 / ATCC29347 tert-butyl-alcohol + NAD(P)+ + H2O
-
?
1.14.15.3 methyl tert-butyl ether + NAD(P)H + H+ + O2
-
Pseudomonas putida tert-butyl-alcohol + NAD(P)+ + H2O + formaldehyde
-
?
1.14.15.3 methyl tert-butyl ether + NAD(P)H + H+ + O2
-
Pseudomonas putida GPo1 / ATCC29347 tert-butyl-alcohol + NAD(P)+ + H2O + formaldehyde
-
?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
Pseudomonas putida n-alkanol + NAD(P)+ + H2O
-
?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
Pseudomonas putida GPo1 / ATCC29347 n-alkanol + NAD(P)+ + H2O
-
?
1.14.15.3 tert-amyl methyl ether + NAD(P)H + H+ + O2
-
Pseudomonas putida tert-amyl-alcohol + NAD(P)+ + H2O + formaldehyde
-
?
1.14.15.3 tert-amyl methyl ether + NAD(P)H + H+ + O2
-
Pseudomonas putida GPo1 / ATCC29347 tert-amyl-alcohol + NAD(P)+ + H2O + formaldehyde
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.3 alkane hydroxylase
-
Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.3 NAD(P)H
-
Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.15.3 0.037
-
n-butane inhibits oxidation of methyl tert-butyl ether Pseudomonas putida
1.14.15.3 0.192
-
propane inhibits oxidation of methyl tert-butyl ether Pseudomonas putida