BRENDA - Enzyme Database

Characterization of an inducible chlorophenol O-methyltransferase from Trichoderma longibrachiatum involved in the formation of chloroanisoles and determination of its role in cork taint of wines

Coque, J.J.; Alvarez-Rodriguez, M.L.; Larriba, G.; Appl. Environ. Microbiol. 69, 5089-5095 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.136
5-methyltetrahydrofolic acid
1 mM, activation to 119% of control
Trichoderma longibrachiatum
2.1.1.136
DTT
1 mM + 1 mM PMSF, activation to 147% of control
Trichoderma longibrachiatum
2.1.1.136
glycerol
10%, activation to 122% of control
Trichoderma longibrachiatum
2.1.1.136
PMSF
1 mM + 1 mM DTT, activation to 147% of control
Trichoderma longibrachiatum
General Stability
EC Number
General Stability
Organism
2.1.1.136
freezing of the purified or semipurified enzymic preparation results in almost complete loss of activity
Trichoderma longibrachiatum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.136
Ag+
1 mM, 94% inhibition
Trichoderma longibrachiatum
2.1.1.136
Ca2+
1 mM, 16% inhibition
Trichoderma longibrachiatum
2.1.1.136
Cu2+
1 mM, 96% inhibition
Trichoderma longibrachiatum
2.1.1.136
Hg2+
0.1 mM, 96% inhibition
Trichoderma longibrachiatum
2.1.1.136
S-adenosyl-L-homocysteine
competitive
Trichoderma longibrachiatum
2.1.1.136
Zn2+
0.1 mM,82% inhibition
Trichoderma longibrachiatum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.136
0.1359
-
2,4,6-Trichlorophenol
-
Trichoderma longibrachiatum
2.1.1.136
0.284
-
S-adenosyl-L-methionine
-
Trichoderma longibrachiatum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.136
52500
-
2 * 52500, SDS-PAGE
Trichoderma longibrachiatum
2.1.1.136
112000
-
gel filtration
Trichoderma longibrachiatum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
Trichoderma longibrachiatum
activity is induced by 2,4,6-trichlorophenol
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
?
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
Trichoderma longibrachiatum CECT 20431
activity is induced by 2,4,6-trichlorophenol
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.1.1.136
Trichoderma longibrachiatum
-
CECT 20431
-
2.1.1.136
Trichoderma longibrachiatum CECT 20431
-
CECT 20431
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.1.1.136
-
Trichoderma longibrachiatum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.136
mycelium
-
Trichoderma longibrachiatum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.1.1.136
0.00055
-
-
Trichoderma longibrachiatum
Storage Stability
EC Number
Storage Stability
Organism
2.1.1.136
-20C, 10% glycerol, stable for up to 3 months
Trichoderma longibrachiatum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.1.1.136
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine
10.7% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine
10.7% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3,4-trichlorophenol + S-adenosyl-L-methionine
93.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3,4-trichlorophenol + S-adenosyl-L-methionine
93.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3,6-trichlorophenol + S-adenosyl-L-methionine
55.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3,6-trichlorophenol + S-adenosyl-L-methionine
55.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3-dichlorophenol + S-adenosyl-L-methionine
22.5% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,5-trichlorophenol + S-adenosyl-L-methionine
64.2% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,5-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,6-tribromophenol + S-adenosyl-L-methionine
13% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-tribromoanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
activity is induced by 2,4,6-trichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
60% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
activity is induced by 2,4,6-trichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
60% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4-dibromophenol + S-adenosyl-L-methionine
36.8% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4-dibromoanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4-dichlorophenol + S-adenosyl-L-methionine
-
657589
Trichoderma longibrachiatum
2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,5-dichlorophenol + S-adenosyl-L-methionine
19.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,5-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,6-dichlorophenol + S-adenosyl-L-methionine
10.4% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,6-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2-chlorophenol + S-adenosyl-L-methionine
9.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2-chloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
3,4-dichlorophenol + S-adenosyl-L-methionine
9.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
3,4-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.1.1.136
dimer
2 * 52500, SDS-PAGE
Trichoderma longibrachiatum
Synonyms
EC Number
Synonyms
Commentary
Organism
2.1.1.136
CPOMT
-
Trichoderma longibrachiatum
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.1.1.136
28
-
-
Trichoderma longibrachiatum
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.1.1.136
42
-
54% loss of activity after 30 min, 89% loss of activity after 1 h
Trichoderma longibrachiatum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.136
8.2
8.5
-
Trichoderma longibrachiatum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.136
7
8.5
pH 7.0: about 50% of maximal activity, pH 7.0-8.5: optimum
Trichoderma longibrachiatum
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.136
0.3789
-
S-adenosyl-L-homocysteine
-
Trichoderma longibrachiatum
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.1.1.136
Trichoderma longibrachiatum
chromatofocusing pH-range 4-7
-
4.9
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.1.1.136
5-methyltetrahydrofolic acid
1 mM, activation to 119% of control
Trichoderma longibrachiatum
2.1.1.136
DTT
1 mM + 1 mM PMSF, activation to 147% of control
Trichoderma longibrachiatum
2.1.1.136
glycerol
10%, activation to 122% of control
Trichoderma longibrachiatum
2.1.1.136
PMSF
1 mM + 1 mM DTT, activation to 147% of control
Trichoderma longibrachiatum
General Stability (protein specific)
EC Number
General Stability
Organism
2.1.1.136
freezing of the purified or semipurified enzymic preparation results in almost complete loss of activity
Trichoderma longibrachiatum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.136
Ag+
1 mM, 94% inhibition
Trichoderma longibrachiatum
2.1.1.136
Ca2+
1 mM, 16% inhibition
Trichoderma longibrachiatum
2.1.1.136
Cu2+
1 mM, 96% inhibition
Trichoderma longibrachiatum
2.1.1.136
Hg2+
0.1 mM, 96% inhibition
Trichoderma longibrachiatum
2.1.1.136
S-adenosyl-L-homocysteine
competitive
Trichoderma longibrachiatum
2.1.1.136
Zn2+
0.1 mM,82% inhibition
Trichoderma longibrachiatum
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1.1.136
0.3789
-
S-adenosyl-L-homocysteine
-
Trichoderma longibrachiatum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.136
0.1359
-
2,4,6-Trichlorophenol
-
Trichoderma longibrachiatum
2.1.1.136
0.284
-
S-adenosyl-L-methionine
-
Trichoderma longibrachiatum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.136
52500
-
2 * 52500, SDS-PAGE
Trichoderma longibrachiatum
2.1.1.136
112000
-
gel filtration
Trichoderma longibrachiatum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
Trichoderma longibrachiatum
activity is induced by 2,4,6-trichlorophenol
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
?
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
Trichoderma longibrachiatum CECT 20431
activity is induced by 2,4,6-trichlorophenol
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.136
-
Trichoderma longibrachiatum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.136
mycelium
-
Trichoderma longibrachiatum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.1.1.136
0.00055
-
-
Trichoderma longibrachiatum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.1.1.136
-20C, 10% glycerol, stable for up to 3 months
Trichoderma longibrachiatum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.1.1.136
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine
10.7% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3,4,5-tetrachlorophenol + S-adenosyl-L-methionine
10.7% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,4,5-tetrachloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3,4-trichlorophenol + S-adenosyl-L-methionine
93.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3,4-trichlorophenol + S-adenosyl-L-methionine
93.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,4-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3,6-trichlorophenol + S-adenosyl-L-methionine
55.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3,6-trichlorophenol + S-adenosyl-L-methionine
55.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,3,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,3-dichlorophenol + S-adenosyl-L-methionine
22.5% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,5-trichlorophenol + S-adenosyl-L-methionine
64.2% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,5-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,6-tribromophenol + S-adenosyl-L-methionine
13% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-tribromoanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
activity is induced by 2,4,6-trichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
60% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
activity is induced by 2,4,6-trichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4,6-trichlorophenol + S-adenosyl-L-methionine
60% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum CECT 20431
2,4,6-trichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4-dibromophenol + S-adenosyl-L-methionine
36.8% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,4-dibromoanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,4-dichlorophenol + S-adenosyl-L-methionine
-
657589
Trichoderma longibrachiatum
2,3-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,5-dichlorophenol + S-adenosyl-L-methionine
19.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,5-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2,6-dichlorophenol + S-adenosyl-L-methionine
10.4% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2,6-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
2-chlorophenol + S-adenosyl-L-methionine
9.6% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
2-chloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
2.1.1.136
3,4-dichlorophenol + S-adenosyl-L-methionine
9.3% of the O-methylation activity with 2,3-dichlorophenol
657589
Trichoderma longibrachiatum
3,4-dichloroanisole + S-adenosyl-L-homocysteine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.1.1.136
dimer
2 * 52500, SDS-PAGE
Trichoderma longibrachiatum
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.1.1.136
28
-
-
Trichoderma longibrachiatum
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.1.1.136
42
-
54% loss of activity after 30 min, 89% loss of activity after 1 h
Trichoderma longibrachiatum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.136
8.2
8.5
-
Trichoderma longibrachiatum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.136
7
8.5
pH 7.0: about 50% of maximal activity, pH 7.0-8.5: optimum
Trichoderma longibrachiatum
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.1.1.136
Trichoderma longibrachiatum
chromatofocusing pH-range 4-7
-
4.9