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Literature summary extracted from
- Characterization of the corrinoid iron-sulfur protein tetrachloroethene reductive dehalogenase of Dehalobacter restrictus (2003), Appl. Environ. Microbiol., 69, 4628-4638.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.21.99.5 | expressed in Escherichia coli DH5alpha | Dehalobacter restrictus |
| 1.21.99.5 | expressed in Escherichia coli DH5alpha | Desulfitobacterium hafniense |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | 1-Iodopropane | - |
Dehalobacter restrictus |  |
| 1.21.99.5 | 1-Iodopropane | - |
Desulfitobacterium hafniense | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.21.99.5 | 0.02 | - |
tetrachloroethene | 30°C | Dehalobacter restrictus | |
| 1.21.99.5 | 0.024 | - |
reduced methyl viologen | 30°C | Dehalobacter restrictus | |
| 1.21.99.5 | 0.047 | - |
trichloroethene | 30°C | Dehalobacter restrictus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.21.99.5 | membrane | - |
Dehalobacter restrictus | 16020 | - |
| 1.21.99.5 | membrane | - |
Desulfitobacterium hafniense | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | Iron | two [4Fe-4S] cluster | Dehalobacter restrictus | |
| 1.21.99.5 | Iron | two [4Fe-4S] cluster | Desulfitobacterium hafniense | |
| 1.21.99.5 | sulfur | two [4Fe-4S] cluster | Dehalobacter restrictus | |
| 1.21.99.5 | sulfur | two [4Fe-4S] cluster | Desulfitobacterium hafniense | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 59000 | - |
SDS-PAGE | Desulfitobacterium hafniense |
| 1.21.99.5 | 59430 | - |
enzyme mass + cobalamin cofactor + iron-sulfur clusters, calculated from deduced amino acid sequence | Dehalobacter restrictus |
| 1.21.99.5 | 60000 | - |
SDS-PAGE | Dehalobacter restrictus |
| 1.21.99.5 | 71000 | - |
gel filtration | Dehalobacter restrictus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.99.5 | tetrachloroethene + 2 reduced acceptor | Dehalobacter restrictus | terminal component of the respiratory chain | cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+ | - |
? | |
| 1.21.99.5 | tetrachloroethene + 2 reduced acceptor | Desulfitobacterium hafniense | terminal component of the respiratory chain | cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.21.99.5 | Dehalobacter restrictus | Q8GJ27 | DSMZ 9455T | - |
| 1.21.99.5 | Desulfitobacterium hafniense | Q8GJ31 | strain TCE1, DSMZ 12704, formerly Desulfitobacterium frappieri strain TCE1 | - |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.21.99.5 | sensitive to oxygen exposure, half-life of 280 h upon exposure to air | Dehalobacter restrictus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.21.99.5 | - |
Dehalobacter restrictus |
| 1.21.99.5 | - |
Desulfitobacterium hafniense |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 13.6 | - |
purified enzyme, 30°C | Dehalobacter restrictus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.99.5 | additional information | trichlofluoroethene, tetrachloromethane, hexachloroethane, tetrachloroethane, trichloroethane, and 1,1,1-trichloro-2,2,2-trifluoroethane cause an enzyme dependent oxidation of reduced methyl viologen | Dehalobacter restrictus | ? | - |
? | |
| 1.21.99.5 | tetrachloroethene + 2 reduced acceptor | terminal component of the respiratory chain | Dehalobacter restrictus | cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+ | - |
? | |
| 1.21.99.5 | tetrachloroethene + 2 reduced acceptor | terminal component of the respiratory chain | Desulfitobacterium hafniense | cis-1,2-dichloroethene + 2 chloride + 2 acceptor + 2 H+ | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced methyl viologen | via trichloroethene | Dehalobacter restrictus | cis-1,2-dichloroethene + 2 chloride + methyl viologen | - |
? | |
| 1.21.99.5 | tetrachloroethene + reduced methyl viologen | via trichloroethene | Desulfitobacterium hafniense | cis-1,2-dichloroethene + 2 chloride + methyl viologen | - |
? | |
| 1.21.99.5 | trichloroethene + chloride + methyl viologen | - |
Dehalobacter restrictus | tetrachloroethene + reduced methyl viologen | - |
? | |
| 1.21.99.5 | trichloroethene + chloride + methyl viologen | - |
Desulfitobacterium hafniense | tetrachloroethene + reduced methyl viologen | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.21.99.5 | monomer | 1 * 60000, SDS-PAGE, native mass of 71000 determined by gel filtration | Dehalobacter restrictus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 8.1 | - |
- |
Dehalobacter restrictus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.21.99.5 | 7.5 | 8.5 | - |
Dehalobacter restrictus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.21.99.5 | Cobalamin | - |
Dehalobacter restrictus | |
| 1.21.99.5 | corrinoid | - |
Desulfitobacterium hafniense | |
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