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Literature summary extracted from

  • Blumel, S.; Knackmuss, H.J.; Stolz, A.
    Molecular cloning and characterization of the gene coding for the aerobic azoreductase from Xenophilus azovorans KF46F (2002), Appl. Environ. Microbiol., 68, 3948-3955.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.7.1.6 environmental protection potential for the treatment of azo dye contaminated wastewater Xenophilus azovorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.6 expressed in Escherichia coli BL21(DE3)pLysS using a phage T7-promotor system, no in vivo activity in recombinant strains due to missing azo dye uptake system Xenophilus azovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.6 20000
-
 gel filtration, differences in molecular weights are supposed to be due to an extraordinary behavior of the enzyme in gel filtration Xenophilus azovorans
1.7.1.6 30000
-
 SDS-PAGE Xenophilus azovorans
1.7.1.6 30280
-
 calculated from the deduced amino acid sequence Xenophilus azovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.6 1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H Xenophilus azovorans substrate can be utilized as sole carbon and energy source 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H Xenophilus azovorans KF46F / DSM 13620 substrate can be utilized as sole carbon and energy source 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Xenophilus azovorans Q8KU07 formerly Pseudomonas sp. KF46F
-
1.7.1.6 Xenophilus azovorans KF46F / DSM 13620 Q8KU07 formerly Pseudomonas sp. KF46F
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6
-
 Xenophilus azovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.6 10.8
-
 purified enzyme, pH 7.1 with 1-(4'-carboxyphenylazo)-2-naphthol and NADH as substrates Xenophilus azovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H substrate can be utilized as sole carbon and energy source Xenophilus azovorans 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H substrate is carboxy-Orange II Xenophilus azovorans 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H substrate can be utilized as sole carbon and energy source Xenophilus azovorans KF46F / DSM 13620 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H substrate is carboxy-Orange II Xenophilus azovorans KF46F / DSM 13620 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.6 monomer 1 * 30000, SDS-PAGE Xenophilus azovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 additional information flavin-free enzyme Xenophilus azovorans
1.7.1.6 NADH
-
 Xenophilus azovorans
1.7.1.6 NADPH preferred over NADH Xenophilus azovorans