EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.7 | crystallized as pure enzyme and in complex with its substrates carnitine and CoA | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.137 | R518N | site-directed mutagenesis, mutant shows 1650fold increased Km for L-carnitine, only slightly affected Km for acyl-CoA and kcat compared to the wild-type enzyme | Bos taurus |
2.3.1.137 | S552A | site-directed mutagenesis, mutant shows 17fold increased Km for L-carnitine compared to the wild-type enzyme | Bos taurus |
2.3.1.137 | S554X | site-directed mutagenesis, mutant shows 10fold decreased kcat compared to the wild-type enzyme | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.137 | membrane | - |
Bos taurus | 16020 | - |
2.3.1.137 | mitochondrion | - |
Bos taurus | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.7 | acetyl-CoA + carnitine | Mus musculus | modulates acetyl-CoA and CoA availability for a wide range of acyl-transfer reactions, involved in fatty acid metabolism | CoA + O-acetylcarnitine | - |
r | |
2.3.1.137 | octanoyl-CoA + L-carnitine | Bos taurus | step in the transport of fatty acids across the inner mitochondrial membrane, reaction plays a role in balancing cellular CoA:acyl-CoA ratio | CoA + L-octanoylcarnitine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.7 | Mus musculus | - |
- |
- |
2.3.1.137 | Bos taurus | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.7 | acetyl-CoA + carnitine = CoA + O-acetylcarnitine | reaction mechanism | Mus musculus | |
2.3.1.137 | octanoyl-CoA + L-carnitine = CoA + L-octanoylcarnitine | catalytic mechanism, active site structure, substrate recognition mechanism, amino acid residues 343, 564 and 553 determine the acyl-CoA substrate specificity, catalytic residue is His343 | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.7 | acetyl-CoA + carnitine | - |
Mus musculus | CoA + O-acetylcarnitine | - |
r | |
2.3.1.7 | acetyl-CoA + carnitine | modulates acetyl-CoA and CoA availability for a wide range of acyl-transfer reactions, involved in fatty acid metabolism | Mus musculus | CoA + O-acetylcarnitine | - |
r | |
2.3.1.137 | octanoyl-CoA + L-carnitine | step in the transport of fatty acids across the inner mitochondrial membrane, reaction plays a role in balancing cellular CoA:acyl-CoA ratio | Bos taurus | CoA + L-octanoylcarnitine | - |
r | |
2.3.1.137 | octanoyl-CoA + L-carnitine | carnitine binding site structure involving the highly conserved R518, Y452 and T465, CoA binding site n the active site tunnel via His343, fatty acid binding site | Bos taurus | CoA + L-octanoylcarnitine | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.137 | carnitine octanoyltransferase | - |
Bos taurus |
2.3.1.137 | CrOT | - |
Bos taurus |
2.3.1.137 | More | enzyme belongs to the family of carnitine acyltransferases | Bos taurus |