Literature summary extracted from

Fraisse, L.; Bonnet, M.C.; de Farcy, J.P.; Agut, C.; Dersigny, D.; Bayol, A.
A colorimetric 96-well microtiter plate assay for the determination of urate oxidase activity and its kinetic parameters (2002), Anal. Biochem., 309, 173-179.

Application

EC Number	Application	Comment	Organism
1.7.3.3	analysis	a colorimetric 96-well microtiter plate assay for the determination of urate oxidase activity and its kinetic parameters based on hydrogen peroxide quantitation. The general advantages of the colorimetric assay are easy handling of large amounts of samples at the same time, the possibility of automation, and the need for less material	Aspergillus flavus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.7.3.3	0.0472	-	Urate	Rasburicase	Aspergillus flavus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.7.3.3	Aspergillus flavus	Q00511	expressed in Saccharomyces cerevisiae	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.7.3.3	urate + O2 + H2O	-	Aspergillus flavus	5-hydroxyisourate + H2O2	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.7.3.3	ELITEK	-	Aspergillus flavus
1.7.3.3	Rasburicase	-	Aspergillus flavus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.7.3.3	18.1	-	Urate	Rasburicase	Aspergillus flavus

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Release 2023.1 (January 2023)