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Literature summary extracted from

  • Bu, W.; Settembre, E.C.; el Kouni, M.H.; Ealick, S.E.
    Structural basis for inhibition of Escherichia coli uridine phosphorylase by 5-substituted acyclouridines (2005), Acta Crystallogr. Sect. D, 61, 863-872.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.3 hanging-drop vapour-diffusion method, structures of 5-benzylacyclouridine, 5-phenylthioacyclouridine, 5-phenylselenylacyclouridine, 5-m-benzyloxybenzyl acyclouridine and 5-m-benzyloxybenzyl barbituric acid acyclonucleoside bound to the active site of the enzyme, resolutions ranging from 1.95 to 2.3 A Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.3 5-benzylacyclouridine
-
 Escherichia coli
2.4.2.3 5-m-benzyloxybenzyl acyclouridine
-
 Escherichia coli
2.4.2.3 5-m-benzyloxybenzyl barbituric acid acyclonucleoside
-
 Escherichia coli
2.4.2.3 5-phenylselenenylacyclouridine
-
 Escherichia coli
2.4.2.3 5-phenylthioacyclouridine
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.3 uridine + phosphate Escherichia coli key enzyme in pyrimidine-salvage pathway uracil + alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Escherichia coli P12758
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 uridine + phosphate
-
 Escherichia coli uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate key enzyme in pyrimidine-salvage pathway Escherichia coli uracil + alpha-D-ribose 1-phosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.3 0.00068
-
 5-m-benzyloxybenzyl acyclouridine
-
 Escherichia coli
2.4.2.3 0.0043
-
 5-benzylacyclouridine
-
 Escherichia coli