Literature summary extracted from

Retailleau, P.; Colloc'h, N.; Vivares, D.; Bonnete, F.; Castro, B.; El Hajji, M.; Prange, T.
Urate oxidase from Aspergillus flavus: new crystal-packing contacts in relation to the content of the active site (2005), Acta Crystallogr. Sect. D, 61, 218-229.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.7.3.3	expression in Saccharomyces cerevisiae	Aspergillus flavus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.7.3.3	sitting-drop vapour-difusion method. Four different crystal forms of Uox are analyzed. In the presence of uracil and 5,6-diaminouracil crystals usually belong to the trigonal space group P3(1)21, the asymmetric unit of which contains one tetramer of Uox. Chemical oxidation of 5,6-diaminouracil within the protein may occur, leading to the canonical (I222) packing with one subunit per asymmetric unit. Coexistence of two crystal forms, P2(1) with two tetramers per asymmetric unit and I222, is found in the same crystallization drop containing another inhibitor, guanine. A fourth form, P2(1)2(1)2 with one tetramer per asymmetric unit, results in the presence of cymelarsan, an additive	Aspergillus flavus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.7.3.3	Aspergillus flavus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.7.3.3	recombinant enzyme	Aspergillus flavus

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Release 2023.1 (January 2023)