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Literature summary extracted from

  • Nok, A.J.; Nzelibe, H.C.; Yako, S.K.
    Trypanosoma evansi sialidase: surface localization, properties and hydrolysis of ghost red blood cells and brain cells-implications in trypanosomiasis (2003), Z. Naturforsch. C, 58, 594-601.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.18 2-Deoxy-2,3-didehydro-N-acetylneuraminic acid i.e. NeuAc2,3en, competitive Trypanosoma evansi
3.2.1.18 Cu2+
-
 Trypanosoma evansi
3.2.1.18 N-bromosuccinimide competitive Trypanosoma evansi
3.2.1.18 N-ethylmaleimide competitive Trypanosoma evansi
3.2.1.18 p-chloromercuribenzoate 50% inhibition at 0.065 mM Trypanosoma evansi
3.2.1.18 Zn2+
-
 Trypanosoma evansi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.18 0.00017
-
 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid pH 6.5, 37°C Trypanosoma evansi
3.2.1.18 0.0029
-
 fetuin pH 5.5, 37°C Trypanosoma evansi
3.2.1.18 0.0049
-
 alpha(2-3)-sialyllactose pH 6.5, 37°C Trypanosoma evansi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.18 plasma membrane
-
 Trypanosoma evansi 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.18 Mg2+ activates Trypanosoma evansi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 Trypanosoma evansi
-
 isolated from blood of infected camels, STIB 731-AA, IL 1392
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.18 glycoprotein
-
 Trypanosoma evansi

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18 310fold by solubilization with Triton CF 54 and ion exchange and fetuin-affinity chromatographic steps Trypanosoma evansi

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.18 174
-
 purified enzyme Trypanosoma evansi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
 Trypanosoma evansi 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
 ?
3.2.1.18 alpha(2-3)-sialyllactose + H2O
-
 Trypanosoma evansi sialic acid + lactose
-
 ?
3.2.1.18 alpha(2-6)-sialyllactose + H2O
-
 Trypanosoma evansi sialic acid + lactose
-
 ?
3.2.1.18 colomic acid + H2O low activity Trypanosoma evansi sialic acid + lactose
-
 ?
3.2.1.18 fetuin + H2O
-
 Trypanosoma evansi sialic acid + ?
-
 ?
3.2.1.18 ganglioside GM2 + H2O
-
 Trypanosoma evansi sialic acid + ?
-
 ?
3.2.1.18 mucin + H2O
-
 Trypanosoma evansi sialic acid + ?
-
 ?
3.2.1.18 Neu5,9-diAc(2-3)-alpha-lactose + H2O low activity Trypanosoma evansi ?
-
 ?
3.2.1.18 red blood ghost cells + H2O desialylation, preference for different species: in descending order mouse, rat, camel, goat, and dog Trypanosoma evansi ?
-
 ?
3.2.1.18 transferrin + H2O
-
 Trypanosoma evansi sialic acid + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.18 sialidase
-
 Trypanosoma evansi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.18 37
-
-
 Trypanosoma evansi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.18 6.5
-
-
 Trypanosoma evansi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.18 0.0053
-
 2-Deoxy-2,3-didehydro-N-acetylneuraminic acid pH 6.5, 37°C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid Trypanosoma evansi
3.2.1.18 0.04
-
 p-chloromercuribenzoate pH 6.5, 37°C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid Trypanosoma evansi
3.2.1.18 0.042
-
 N-bromosuccinimide pH 6.5, 37°C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid Trypanosoma evansi
3.2.1.18 0.053
-
 N-ethylmaleimide pH 6.5, 37°C, versus 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid Trypanosoma evansi