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Literature summary extracted from

  • Stich, K.; Halbwirth, H.; Wurst, F.; Forkmann, G.
    UDP-glucose:flavonol 7-O-glucosyltransferase activity in flower extracts of Chrysanthemum segetum (1997), Z. Naturforsch. C, 52, 153-158.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.237 bovine serum albumin addition to the assay system causes 2.5fold higher reaction rate Glebionis segetum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.237 Co2+ 1 mM, 98% inhibition with quercetin as substrate, 85% inhibition with gossypetin as substrate Glebionis segetum
2.4.1.237 Cu2+ 1 mM, 99% inhibition with quercetin as substrate Glebionis segetum
2.4.1.237 diethyldicarbonate 5 mM, 98% inhibition with quercetin or gossypetin as substrate Glebionis segetum
2.4.1.237 Fe2+ 1 mM, 98% inhibition with quercetin as substrate Glebionis segetum
2.4.1.237 Hg2+ 1 mM, 99% inhibition with quercetin as substrate Glebionis segetum
2.4.1.237 additional information not inhibited by EDTA Glebionis segetum
2.4.1.237 N-ethylmaleimide strong, 1 mM, 98% inhibition with quercetin as substrate, 14 mM 2-mercaptoethanol protects, but addition after 15 min does not restore activity Glebionis segetum
2.4.1.237 p-hydroxymercuribenzoate strong, 0.1 mM, 99.3% inhibition with quercetin as substrate, 14 mM 2-mercaptoethanol protects and addition after 15 min also reduces the inhibitory effect Glebionis segetum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.237 0.029
-
 kaempferol pH 7.8, 25°C Glebionis segetum
2.4.1.237 0.032
-
 gossypetin pH 6.9, 25°C Glebionis segetum
2.4.1.237 0.049
-
 quercetin pH 8.3, 25°C Glebionis segetum
2.4.1.237 0.067
-
 myricetin pH 8.1, 25°C Glebionis segetum
2.4.1.237 0.071
-
 eriodictyol pH 7.5, 25°C Glebionis segetum
2.4.1.237 0.073
-
 naringenin pH 6.9, 25°C Glebionis segetum
2.4.1.237 0.078
-
 apigenin pH 7.7, 25°C Glebionis segetum
2.4.1.237 0.084
-
 luteolin pH 8.1, 25°C Glebionis segetum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.237 Ca2+ slight stimulation Glebionis segetum
2.4.1.237 KCN slight stimulation Glebionis segetum
2.4.1.237 Mg2+ slight stimulation Glebionis segetum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.237 UDP-alpha-D-glucose + quercetin Glebionis segetum gossypetin and quercetin are presumably the natural substrates UDP + quercetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + gossypetin Glebionis segetum gossypetin and quercetin are presumably the natural substrates UDP + gossypetin 7-O-beta-D-glucoside gossypetin 7-O-glucoside is particularly responsible for the yellow coloration of the petals ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.237 Glebionis segetum
-
 cv. Prado and Stern des Orients
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.237 UDP-glucose + a flavonol = UDP + a flavonol 7-O-beta-D-glucoside acts on the flavonols gossypetin (8-hydroxyquercetin) and to a lesser extend on quercetin, kaempferol and myricetin Glebionis segetum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.237 petal
-
 Glebionis segetum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.237 additional information
-
 specific activities for the 7-O-glucosylation reaction with several substrates, cv. Stern des Orients: 3times higher specific activity with gossypetin than with quercetin as substrate Glebionis segetum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.237 UDP-alpha-D-glucose + kaempferol cv. Prado, gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin Glebionis segetum UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-alpha-D-glucose + quercetin gossypetin and quercetin are presumably the natural substrates Glebionis segetum UDP + quercetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-alpha-D-glucose + quercetin cv. Prado: gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin, cv. Stern des Orients: 3times lower specific activity than with gossypetin as substrate Glebionis segetum UDP + quercetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + a flavonol
-
 Glebionis segetum UDP + a flavonol 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + apigenin cv. Prado Glebionis segetum UDP + apigenin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + eriodictyol cv. Prado Glebionis segetum UDP + eriodictyol 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + gossypetin gossypetin and quercetin are presumably the natural substrates Glebionis segetum UDP + gossypetin 7-O-beta-D-glucoside gossypetin 7-O-glucoside is particularly responsible for the yellow coloration of the petals ?
2.4.1.237 UDP-glucose + gossypetin cv. Prado: best substrate, followed by quercetin, kaempferol and myricetin, cv. Stern des Orients: 3times higher specific activity than with quercetin as substrate Glebionis segetum UDP + gossypetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + luteolin cv. Prado Glebionis segetum UDP + luteolin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + myricetin cv. Prado, gossypetin is the best substrate, followed by quercetin, kaempferol and myricetin Glebionis segetum UDP + myricetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + naringenin cv. Prado Glebionis segetum UDP + naringenin 7-O-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.237 UDP-glucose:flavonol 7-O-glucosyltransferase
-
 Glebionis segetum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.237 25
-
-
 Glebionis segetum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.237
-
-
 7-O-glucosylation also proceeds at low temperatures, e.g. 0°C Glebionis segetum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.237 40
-
 10 min, no influence on the reaction up to 40°C, higher temperatures lead to loss of activity Glebionis segetum
2.4.1.237 60
-
 10 min, incubation without UDP-glucose, complete loss of activity Glebionis segetum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.237 additional information
-
 the pH-optimum is highly dependent on the substrate used Glebionis segetum
2.4.1.237 6.9
-
 gossypetin or naringenin as substrate Glebionis segetum
2.4.1.237 7.5
-
 eriodictyol as substrate Glebionis segetum
2.4.1.237 7.7
-
 apigenin as substrate Glebionis segetum
2.4.1.237 7.8
-
 kaempferol as substrate Glebionis segetum
2.4.1.237 8.1
-
 myricetin or luteolin as substrate Glebionis segetum
2.4.1.237 8.3
-
 quercetin as substrate Glebionis segetum