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Literature summary extracted from

  • Omidinia, E.; Samadi, A.; Taherkhani, H.; Khatami, S.; Moazami, N.; Pouraie, R.R.; Asano, Y.
    Cloning and expression of Bacillus sphaericus phenylalanine dehydrogenase gene in Bacillus subtilis cells: purification and enzyme properties (2002), World J. Microbiol. Biotechnol., 18, 593-597.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.20 expression in Bacillus subtilis Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.20 0.19
-
 NAD+
-
 Lysinibacillus sphaericus
1.4.1.20 0.24
-
 L-phenylalanine
-
 Lysinibacillus sphaericus
1.4.1.20 0.48
-
 L-tyrosine
-
 Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.20 41000
-
 8 * 41000, SDS-PAGE Lysinibacillus sphaericus
1.4.1.20 340000
-
 gel filtration Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.20 L-phenylalanine + H2O + NAD+ Lysinibacillus sphaericus
-
 phenylpyruvate + NH3 + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.20 recombinant PheDH, ammonium sulfate, DEAE-Toyopearl, hydroxyapatite, Sephadex G-200 Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.20 285.4
-
 recombinant PheDH, oxidative deamination Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 L-leucine + H2O + NAD+ 1% of activity with L-phenylalanine Lysinibacillus sphaericus 3,3-dimethyl-2-oxo-butanoate + NH3 + NADH
-
 r
1.4.1.20 L-methionine + H2O + NAD+ 3% of activity with L-phenylalanine Lysinibacillus sphaericus (4-methylsulfanyl)-2-oxobutanoate + NH3 + NADH
-
 r
1.4.1.20 L-norleucine + H2O + NAD+ 4.5% of activity with L-phenylalanine Lysinibacillus sphaericus 2-oxohexanoate + NH3 + NADH
-
 r
1.4.1.20 L-phenylalanine + H2O + NAD+
-
 Lysinibacillus sphaericus phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-tryptophan + H2O + NAD+ 2% of activity with L-phenylalanine Lysinibacillus sphaericus 3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + NADH
-
 r
1.4.1.20 L-tyrosine + H2O + NAD+ 74% of activity with L-phenylalanine Lysinibacillus sphaericus (4-hydroxyphenyl)pyruvate + NH3 + NADH
-
 r
1.4.1.20 L-valine + H2O + NAD+ 1.8% of activity with L-phenylalanine Lysinibacillus sphaericus 3-methyl-2-oxobutanoate + NH3 + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.20 octamer 8 * 41000, SDS-PAGE Lysinibacillus sphaericus

Synonyms

EC Number Synonyms Comment Organism
1.4.1.20 PheDH
-
 Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.20 10.2
-
 reductive amination Lysinibacillus sphaericus
1.4.1.20 11
-
 oxidative deamination Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 NADH
-
 Lysinibacillus sphaericus