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Literature summary extracted from

  • Tao, X.; Yang, Z.; Tong, L.
    Crystal structures of substrate complexes of malic enzyme and insights into the catalytic mechanism (2003), Structure, 11, 1141-1150.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.39 fumarate allosteric activator Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.39 enzyme complexed with the natural substrate malate or pyruvate, NAD+ or NADH, Mn2+, and the allosteric activator fumarate, X-ray diffraction structure determination and analysis at 2.1 A resolution, structure modeling Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.39 mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.39 Mn2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.39 (S)-malate + NAD(P)+ Homo sapiens
-
 pyruvate + CO2 + NAD(P)H
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.39 (S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ catalytic mechanism, malate is bound deeply in the active site, Mn2+ catalyzes the entire reaction, Lys183 is the general base for oxidation, Tyr112-Lys183 functions as the general acid-base pair to catalyze the tautomerization of the enolpyruvate product from decarboxylation to pyruvate, substrate and cofacor binding modes Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 (S)-malate + NAD(P)+
-
 Homo sapiens pyruvate + CO2 + NAD(P)H
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.39 malic enzyme
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+
-
 Homo sapiens
1.1.1.39 NADH
-
 Homo sapiens
1.1.1.39 NADP+
-
 Homo sapiens
1.1.1.39 NADPH
-
 Homo sapiens