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Literature summary extracted from

  • Qujeq, D.
    Development of a quantitative assay method for 3 beta-hydroxy-DELTA 5-steroid dehydrogenase in the rat testis (2002), Steroids, 67, 1071-1077.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.0000047
-
 NAD+ 37°C, pH 7.25 Rattus norvegicus
1.1.1.145 0.00047
-
 pregnenolone 37°C, pH 7.25 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 5-pregnen-3beta-ol-2-one + NAD+ Rattus norvegicus trivial name pregnenolone progesterone + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 5-pregnen-3beta-ol-2-one + NAD+ trivial name pregnenolone Rattus norvegicus progesterone + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.145 37
-
-
 Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.145 5 65 approx. 50% of maximal activity at 20°C and 45°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.145 7.25
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.145 2 12 approx. 50% of maximal activity at pH 4.0 and pH 10.0 Rattus norvegicus