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Literature summary extracted from

  • Lama, L.; Calandrelli, V.; Gambacorta, A.; Nicolaus, B.
    Purification and characterization of thermostable xylanase and beta-xylosidase by the thermophilic bacterium Bacillus thermantarcticus (2004), Res. Microbiol., 155, 283-289.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+ 1 mM, 85% residual activity Parageobacillus thermantarcticus
3.2.1.8 FeSO4 1 mM, 61% residual activity Parageobacillus thermantarcticus
3.2.1.8 N-bromosuccinimide 1 mM, 59% residual activity Parageobacillus thermantarcticus
3.2.1.8 sodium dodecylsulfate 1 mM, 95% residual activity Parageobacillus thermantarcticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.5
-
 p-nitrophenyl-beta-D-xylopyranoside pH 6.0, 70°C Parageobacillus thermantarcticus
3.2.1.37 1.28
-
 o-nitrophenyl-beta-D-xylopyranoside pH 6.0, 70°C Parageobacillus thermantarcticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Parageobacillus thermantarcticus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 45000
-
 * 45000, SDS-PAGE Parageobacillus thermantarcticus
3.2.1.37 75000
-
 2 * 75000, SDS-PAGE Parageobacillus thermantarcticus
3.2.1.37 150000
-
 gel filtration Parageobacillus thermantarcticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Parageobacillus thermantarcticus
-
-
-
3.2.1.37 Parageobacillus thermantarcticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Parageobacillus thermantarcticus
3.2.1.37
-
 Parageobacillus thermantarcticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 141
-
 pH 5.6, 80°C Parageobacillus thermantarcticus
3.2.1.37 160
-
 pH 6.0, 70°C Parageobacillus thermantarcticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 xylan + H2O
-
 Parageobacillus thermantarcticus ?
-
 ?
3.2.1.37 o-nitrophenyl-beta-D-xylopyranoside + H2O
-
 Parageobacillus thermantarcticus o-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 p-nitrophenyl-beta-D-xylopyranoside + H2O
-
 Parageobacillus thermantarcticus p-nitrophenol + beta-D-xylopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? * 45000, SDS-PAGE Parageobacillus thermantarcticus
3.2.1.37 dimer 2 * 75000, SDS-PAGE Parageobacillus thermantarcticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 80
-
-
 Parageobacillus thermantarcticus
3.2.1.37 70
-
-
 Parageobacillus thermantarcticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 60
-
 stable for 24 h Parageobacillus thermantarcticus
3.2.1.8 70
-
 half-life 24 h Parageobacillus thermantarcticus
3.2.1.8 80
-
 hlaf-life 50 min Parageobacillus thermantarcticus
3.2.1.37 60
-
 1 h, stable Parageobacillus thermantarcticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5.6
-
-
 Parageobacillus thermantarcticus
3.2.1.37 6
-
-
 Parageobacillus thermantarcticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Parageobacillus thermantarcticus isoelectric focusing
-
 4.8
3.2.1.37 Parageobacillus thermantarcticus
-
-
 4.2