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Literature summary extracted from

  • Pritchard, D.G.; Trent, J.O.; Zhang, P.; Egan, M.L.; Baker, J.R.
    Characterization of the active site of group B streptococcal hyaluronan lyase (2000), Proteins Struct. Funct. Genet., 40, 126-134.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.1 DNA sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli Streptococcus agalactiae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.1 A425K site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 A487S site-directed mutagenesis, unaltered activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 D170A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 D170E site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 D373H site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 D373H/K466Y/D478Q site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 D432A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 D473E site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 D473G site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 D478Q site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 D494T site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 D494T/K466Y site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 D537A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 E366S site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 E366S/G367R site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 E440V site-directed mutagenesis, mutation of a residue in the putative EF hand motif, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 G367R site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 G434A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 G541A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 H479A site-directed mutagenesis, inactive mutant Streptococcus agalactiae
4.2.2.1 H479G site-directed mutagenesis, inactive mutant Streptococcus agalactiae
4.2.2.1 I544D site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 K437A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 K437A/R542A site-directed mutagenesis, mutation of residues in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 K466Y site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 K466Y/D478Q site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 K466Y/D478Q/K725L/T726W site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 K725L/T726W site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 K861E site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 M659G/D661K site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 M659G/D661K/K725L/T726W site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 N312A site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 N370A site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 N429A site-directed mutagenesis, inactive mutant Streptococcus agalactiae
4.2.2.1 N660A site-directed mutagenesis, inactive mutant Streptococcus agalactiae
4.2.2.1 R321L/R322Q site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 R380Q site-directed mutagenesis, unaltered activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 R380Q/A478S site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 R540A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 R542A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 R546A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 S539A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, unaltered activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 S545V site-directed mutagenesis, mutation of a residue in the putative EF hand motif, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 W371A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 W371A/W372A site-directed mutagenesis, inactive mutant Streptococcus agalactiae
4.2.2.1 W372A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 Y484F site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
4.2.2.1 Y488F site-directed mutagenesis, inactive mutant Streptococcus agalactiae
4.2.2.1 Y488L site-directed mutagenesis, inactive mutant Streptococcus agalactiae
4.2.2.1 Y488T site-directed mutagenesis, inactive mutant Streptococcus agalactiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.1 2,3-Butanedione inactivation, arginine-specific reagent Streptococcus agalactiae
4.2.2.1 Tetranitromethane inactivation, tyrosine-specific reagent Streptococcus agalactiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.1 Ca2+ activates Streptococcus agalactiae

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.1 Streptococcus agalactiae
-
 serotype III GBS strain 3502
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.1 recombinant wild-type and mutant enzymes from Escherichia coli Streptococcus agalactiae

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.1 [hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate His479 is critical for activity, active site structure, mechanism Streptococcus agalactiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.1 additional information
-
 activity of wild-type and mutant enzymes in diameter of clear zone produced on HA plates in centimeters Streptococcus agalactiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.1 hyaluronan
-
 Streptococcus agalactiae 2-acetamido-2-deoxy-3-(beta-D-4-deoxy-gluco-4-enepyranosyluronic acid)-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.1 More homology model from crystal structure Streptococcus agalactiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.1 30
-
 assay at Streptococcus agalactiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.1 6.8
-
 assay at Streptococcus agalactiae