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Literature summary extracted from

  • Fitzpatrick, T.B.; Auweter, S.; Kitzing, K.; Clausen, T.; Amrhein, N.; Macheroux, P.
    Structural and functional impairment of an Old Yellow Enzyme homologue upon affinity tag incorporation (2004), Protein Expr. Purif., 36, 280-291.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.99.1 0.001
-
 N-ethylmaleimide or below, pH 7.5, 25°C, GST-fusion protein Bacillus subtilis
1.6.99.1 0.001
-
 N-ethylmaleimide or below, pH 7.5, 25°C, native protein Bacillus subtilis
1.6.99.1 0.045
-
 nitroglycerin pH 7.5, 25°C, GST-fusion protein Bacillus subtilis
1.6.99.1 0.109
-
 nitroglycerin pH 7.5, 25°C, native protein Bacillus subtilis
1.6.99.1 0.123
-
 2,4,6-trinitrotoluene pH 7.5, 25°C, GST-fusion protein Bacillus subtilis
1.6.99.1 0.186
-
 cyclohex-2-enone pH 7.5, 25°C, GST-fusion protein Bacillus subtilis
1.6.99.1 0.293
-
 cyclohex-2-enone pH 7.5, 25°C, native protein Bacillus subtilis
1.6.99.1 0.705
-
 2,4,6-trinitrotoluene pH 7.5, 25°C, native protein Bacillus subtilis
1.6.99.1 1.853
-
 trans-hex-2-enal pH 7.5, 25°C, GST-fusion protein Bacillus subtilis
1.6.99.1 2.602
-
 trans-hex-2-enal pH 7.5, 25°C, native protein Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.99.1 37400
-
 4 * 37400, SDS-PAGE, native protein, 4 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium Bacillus subtilis
1.6.99.1 38300
-
 2 * 38300, SDs-PAGE, His-tagged protein, 2 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium Bacillus subtilis
1.6.99.1 62900
-
 2 * 38300, SDs-PAGE, His-tagged protein, 2 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium Bacillus subtilis
1.6.99.1 62900
-
 4 * 37400, SDS-PAGE, native protein, 4 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium Bacillus subtilis
1.6.99.1 76500
-
 gel filtration, His-tagged protein Bacillus subtilis
1.6.99.1 114400
-
 gel filtration, GST-tagged protein Bacillus subtilis
1.6.99.1 147000
-
 gel filtration, native protein Bacillus subtilis
1.6.99.1 247500
-
 gel filtration, GST-tagged protein Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.6.99.1 Bacillus subtilis
-
 native enzyme and expression of N-terminal glutathione-S-transferase fusion and a C-terminal His-tagged form in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.99.1 N-terminal glutathione-S-transferase fusion and a C-terminal His-tagged form expressed in Escherichia coli Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.99.1 NADPH + H+ + 2,4,6-trinitrotoluene
-
 Bacillus subtilis NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + cyclohex-2-enone
-
 Bacillus subtilis NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + N-ethylmaleimide
-
 Bacillus subtilis NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + nitroglycerin
-
 Bacillus subtilis NADP+ + ?
-
 ?
1.6.99.1 NADPH + H+ + trans-hex-2-enal
-
 Bacillus subtilis NADP+ + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.99.1 dimer 2 * 38300, SDs-PAGE, His-tagged protein, 2 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium Bacillus subtilis
1.6.99.1 tetramer 4 * 37400, SDS-PAGE, native protein, 4 * 62900, SDS-PAGE, GST-tagged protein, in 3:1 dimer/tetramer equilibrium Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.6.99.1 YqjM
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.99.1 additional information
-
 additional information half-reaction kinetics of tagged and untagged enzyme Bacillus subtilis
1.6.99.1 0.53
-
 2,4,6-trinitrotoluene pH 7.5, 25°C, GST-fusion protein Bacillus subtilis
1.6.99.1 0.75
-
 N-ethylmaleimide pH 7.5, 25°C, GST-fusion protein Bacillus subtilis
1.6.99.1 0.82
-
 nitroglycerin pH 7.5, 25°C, GST-fusion protein Bacillus subtilis
1.6.99.1 1.17
-
 trans-hex-2-enal pH 7.5, 25°C, GST-fusion protein Bacillus subtilis
1.6.99.1 1.2
-
 cyclohex-2-enone pH 7.5, 25°C, GST-fusion protein Bacillus subtilis
1.6.99.1 3.19
-
 2,4,6-trinitrotoluene pH 7.5, 25°C, native protein Bacillus subtilis
1.6.99.1 3.72
-
 nitroglycerin pH 7.5, 25°C, native protein Bacillus subtilis
1.6.99.1 4.38
-
 cyclohex-2-enone pH 7.5, 25°C, native protein Bacillus subtilis
1.6.99.1 4.68
-
 trans-hex-2-enal pH 7.5, 25°C, native protein Bacillus subtilis
1.6.99.1 8.07
-
 N-ethylmaleimide pH 7.5, 25°C, native protein Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.99.1 NADPH
-
 Bacillus subtilis