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Literature summary extracted from

  • Jin, M.; Rosario, W.; Watler, E.; Calhoun, D.H.
    Development of a large-scale HPLC-based purification for the urease from Staphylococcus leei and determination of subunit structure (2004), Protein Expr. Purif., 34, 111-117.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 1.66
-
 Urea pH 6.8, 37°C Staphylococcus leei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.5 12000
-
 (alpha,beta,gamma)5, 5 * 65000 + 5 * 21000 + 5 * 12000, SDS-PAGE Staphylococcus leei
3.5.1.5 21000
-
 (alpha,beta,gamma)5, 5 * 65000 + 5 * 21000 + 5 * 12000, SDS-PAGE Staphylococcus leei
3.5.1.5 65000
-
 (alpha,beta,gamma)5, 5 * 65000 + 5 * 21000 + 5 * 12000, SDS-PAGE Staphylococcus leei
3.5.1.5 480000
-
 gel filtration Staphylococcus leei

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Staphylococcus leei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5 large-scale HPLC-based Staphylococcus leei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.5 730
-
-
 Staphylococcus leei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Staphylococcus leei CO2 + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.5 pentadecamer (alpha,beta,gamma)5, 5 * 65000 + 5 * 21000 + 5 * 12000, SDS-PAGE Staphylococcus leei