BRENDA - Enzyme Database

Large scale production of biologically active Escherichia coli glutamyl-tRNA reductase from inclusion bodies

Schauer, S.; Luer, C.; Moser, J.; Protein Expr. Purif. 31, 271-275 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.70
expression plasmid pBKCwt overexpression of a 6*His-tagged enzyme
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.70
inclusion body
-
Escherichia coli
16234
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
Escherichia coli
the enzyme catalyzes the initial step of tetrapyrrole biosynthesis in plants and prokaryotes
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.70
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.70
0.47
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
1.2.1.70
-80°C, 6*His-tagged enzyme is stable for at least 6 months
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
657264
Escherichia coli
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
the enzyme catalyzes the initial step of tetrapyrrole biosynthesis in plants and prokaryotes
657264
Escherichia coli
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.70
37
-
-
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.70
8.1
-
-
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADPH
-
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
expression plasmid pBKCwt overexpression of a 6*His-tagged enzyme
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADPH
-
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.70
inclusion body
-
Escherichia coli
16234
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
Escherichia coli
the enzyme catalyzes the initial step of tetrapyrrole biosynthesis in plants and prokaryotes
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.70
0.47
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.1.70
-80°C, 6*His-tagged enzyme is stable for at least 6 months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
657264
Escherichia coli
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
the enzyme catalyzes the initial step of tetrapyrrole biosynthesis in plants and prokaryotes
657264
Escherichia coli
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.70
37
-
-
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.70
8.1
-
-
Escherichia coli