EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.70 | expression plasmid pBKCwt overexpression of a 6*His-tagged enzyme | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.2.1.70 | inclusion body | - |
Escherichia coli | 16234 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.70 | L-glutamyl-tRNAGlu + NADPH + H+ | Escherichia coli | the enzyme catalyzes the initial step of tetrapyrrole biosynthesis in plants and prokaryotes | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.70 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.70 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.70 | 0.47 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.1.70 | -80°C, 6*His-tagged enzyme is stable for at least 6 months | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.70 | L-glutamyl-tRNAGlu + NADPH + H+ | - |
Escherichia coli | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? | |
1.2.1.70 | L-glutamyl-tRNAGlu + NADPH + H+ | the enzyme catalyzes the initial step of tetrapyrrole biosynthesis in plants and prokaryotes | Escherichia coli | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.70 | glutamate tRNA reductase | - |
Escherichia coli |
1.2.1.70 | glutamate-specific tRNA reductase | - |
Escherichia coli |
1.2.1.70 | glutamyl transfer RNA reductase | - |
Escherichia coli |
1.2.1.70 | glutamyl-tRNA reductase | - |
Escherichia coli |
1.2.1.70 | GluTR | - |
Escherichia coli |
1.2.1.70 | reductase, glutamyl-transfer ribonucleate | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.70 | 37 | - |
- |
Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.70 | 8.1 | - |
- |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.70 | NADPH | - |
Escherichia coli |