Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nieuwenhuizen, W.F.; van Leeuwen, S.; Jack, R.W.; Egmond, M.R.; Gotz, F.
    Molecular cloning and characterization of the alkaline ceramidase from Pseudomonas aeruginosa PA01 (2003), Protein Expr. Purif., 30, 94-104.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.23 medicine CDase from Pseudomonas aeruginosa may be a virulence factor in infections, may be involved in the ceramide deficiency in the horny layer of the epidermis of patients with atopic dermatitis suffering from bacterial infections Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 expression in Escherichia coli DH5alpha and BL21, expression at low levels in Pseudomonas putida KT2440 and Pichia pastoris GS115, sequencing Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 D-erythro-sphingosine recombinant CDase, more inhibitory than L-erythro-sphingosine Pseudomonas aeruginosa
3.5.1.23 DL-sphinganine recombinant CDase Pseudomonas aeruginosa
3.5.1.23 EDTA recombinant CDase, complete inhibition, Ca2+ restores activity Pseudomonas aeruginosa
3.5.1.23 EGTA recombinant CDase, complete inhibition, Ca2+ restores activity Pseudomonas aeruginosa
3.5.1.23 L-erythro-sphingosine recombinant CDase, 0.02 mM, 20% inhibition, less inhibitory than L-erythro-sphingosine Pseudomonas aeruginosa
3.5.1.23 additional information not inhibited by 1 mM iodoacetic acid, 0.1 mM 2-mercaptoethanol, 0.05 mM hexadecylsulfonylfluoride, 1 mM p-phenylmethylsulfonylfluoride, 0.2 mM D-erythro-2-(N-myristoylamino)-1-phenyl-1-propanol, 0.5 mM N-oleoylethanolamine, L(-)- and D(-)-norephenedrin Pseudomonas aeruginosa
3.5.1.23 phytosphingosine recombinant CDase, 0.02 mM, 60% inhibition Pseudomonas aeruginosa
3.5.1.23 sphingosine strong inhibitor Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 extracellular wild-type CDase from 3-L culture, recombinant CDase expressed in Pseudomonas putida KT2440 and Pichia pastoris GS115 Pseudomonas aeruginosa
-
-
3.5.1.23 inclusion body inactive recombinant CDase expressed in Escherichia coli BL21 without its signal peptide Pseudomonas aeruginosa 16234
-
3.5.1.23 intracellular recombinant CDase expressed in Escherichia coli DH5alpha and BL21 Pseudomonas aeruginosa 5622
-
3.5.1.23 periplasm recombinant CDase expressed in Escherichia coli DH5alpha is most likely present in periplasm Pseudomonas aeruginosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.23 Ba2+ better activator than Mg2+ Pseudomonas aeruginosa
3.5.1.23 Ca2+ Ca2+-dependent, better activator than Mg2+ Pseudomonas aeruginosa
3.5.1.23 Mg2+ the maximum activity with Mg2+ is only 36% of that in presence of optimal Ca2+ or Ba2+ concentrations Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 70000
-
 x * 70000, wild-type and recombinant CDase, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 additional information Pseudomonas aeruginosa CDase may be a virulence factor ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Pseudomonas aeruginosa Q9I596 strain PA01
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.23 glycoprotein recombinant CDase expressed in Pichia pastoris GS115 Pseudomonas aeruginosa
3.5.1.23 additional information CDase contains a 26 amino acids signal peptide Pseudomonas aeruginosa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 wild-type CDase from 3-L culture: 217fold, recombinant CDase expressed in Escherichia coli Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 cell culture 3-L culture Pseudomonas aeruginosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.23 0.218
-
 intracellular recombinant CDase expressed in Escherichia coli DH5alpha, pH 8.5, hydrolysis of N-palmitoyl sphingosine Pseudomonas aeruginosa
3.5.1.23 0.239
-
 extracellular wild-type CDase, pH 8.5, hydrolysis of N-palmitoyl sphingosine Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 additional information CDase may be a virulence factor Pseudomonas aeruginosa ?
-
 ?
3.5.1.23 N-acylsphingosine + H2O CDase hydrolyzes the amide bond in ceramides to yield free fatty acid and sphingosine Pseudomonas aeruginosa a carboxylate + sphingosine
-
 ?
3.5.1.23 N-palmitoyl sphingosine + H2O
-
 Pseudomonas aeruginosa palmitic acid + sphingosine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.23 ? x * 70000, wild-type and recombinant CDase, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 alkaline ceramidase
-
 Pseudomonas aeruginosa
3.5.1.23 CDase
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 8.5
-
 wild-type and recombinant CDase Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.23 0.0033
-
 DL-sphinganine pH 8.5, recombinant CDase Pseudomonas aeruginosa
3.5.1.23 0.004
-
 D-erythro-sphingosine pH 8.5, recombinant CDase Pseudomonas aeruginosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.23 Pseudomonas aeruginosa recombinant CDase from inclusion bodies, expressed in Escherichia coli BL21 without its signal peptide, isoelectric focusing
-
 4.9
3.5.1.23 Pseudomonas aeruginosa sequence calculation
-
 6